An Outwardly Rectifying and Deactivating Chloride Channel Expressed by Interstitial Cells of Cajal from the Murine Small Intestine

An Outwardly Rectifying and Deactivating Chloride Channel Expressed by Interstitial Cells of... Recent studies have suggested a role for a chloride current in the modulation of pacemaker potentials generated by interstitial cells of Cajal. Patch-clamp recordings were made from inside–out patches of cultured interstitial cells of Cajal from the murine small intestine. The majority of patches were quiescent immediately after excision, but in some patches currents activated spontaneously after a period of 10 min to 1 h. Currents could also be activated by strongly polarizing the patch. It was found that the currents activated in both cases included a chloride channel. This channel could also be activated by ATP and the catalytic subunit of protein kinase A. The channel had conductance states (±SD) of 53 ± 25.35, 126 ± 21.44, 180 ± 12.57 and 211 ± 8.86 pS. It was outwardly rectifying (as a function of open probability) and deactivated (i.e., gave a tail current) but showed no inactivation. The permeability sequence of the channel was I−>>Br−≥Cl−>Asp−. It was unaffected in magnitude or rectification by changing the free Ca2+ concentration of the bath between <10 nm, 100 nm (control) and 2 mm. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Membrane Biology Springer Journals

An Outwardly Rectifying and Deactivating Chloride Channel Expressed by Interstitial Cells of Cajal from the Murine Small Intestine

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Publisher
Springer-Verlag
Copyright
Copyright © 2008 by Springer Science+Business Media, LLC
Subject
Life Sciences; Human Physiology ; Biochemistry, general
ISSN
0022-2631
eISSN
1432-1424
D.O.I.
10.1007/s00232-007-9084-2
Publisher site
See Article on Publisher Site

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