A ﬂuorescent probe 4-amino-7-nitrobenz-2-oxa-1,3-diazole NBD–NH for critical sensing hypochlorous (ClO ) has been developed in this paper. Probe NBD–NH exhibited excellent sensitivity and high selectivity toward detection of ClO in CH CH OH/HEPES solution (1:4, v/v, 10 mM, pH 7.4), where the detection limit as low as 40 nM. The green ﬂuorescence 3 2 of NBD–NH was distinctly quenched by addition of ClO . In addition, the new probe was also applied for bioimaging using HeLa cells and exist a critical detection of ClO in living cells. Graphical abstract NH NO Cl N N NH H O ClO 3' 2 O O N N CH OH NO NO 2 2 NO NBD-Cl NBD-NH NBD-NO Keywords NBD–NH Fluorescent probe Hypochlorous Introduction in living organisms [3–5]. However, excessive or mis- placed generation of HClO always can produce many Hypochlorous acid (HClO)/hypochlorite (ClO ), as one of human diseases or tissue damage. For example, neuron important reactive oxygen species (ROS), play an indis- degeneration can cause atherosclerosis [6–9]. Therefore, it pensable role in varieties of physical and biological sys- is very important to develop different efﬁcient protocols to - - tems [1, 2]. Endogenous ClO usually generated from detect the
Monatshefte für Chemie - Chemical Monthly – Springer Journals
Published: Feb 13, 2018
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