An NA-deficient 2009 pandemic H1N1 influenza virus mutant can efficiently replicate in cultured cells

An NA-deficient 2009 pandemic H1N1 influenza virus mutant can efficiently replicate in cultured... We identified a novel neuraminidase (NA)-deficient virus that was a 2009 pandemic influenza H1N1 virus mutant. The mutant virus had a deletion of 1,009 nt in the NA gene and lacked an enzymatic domain. Although the yield of the NA-deficient virus was limited, it formed large plaques when applied to MDCK cell cultures, indicating that the virus was able to spread to adjacent cells. Furthermore, the NA-deficient virus was eluted from chicken erythrocytes at 37 °C, even in the presence of the antiviral drug peramivir. Spread of this NA-deficient virus may pose a potential threat to anti-influenza therapies. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

An NA-deficient 2009 pandemic H1N1 influenza virus mutant can efficiently replicate in cultured cells

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Publisher
Springer Journals
Copyright
Copyright © 2014 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-013-1887-0
Publisher site
See Article on Publisher Site

Abstract

We identified a novel neuraminidase (NA)-deficient virus that was a 2009 pandemic influenza H1N1 virus mutant. The mutant virus had a deletion of 1,009 nt in the NA gene and lacked an enzymatic domain. Although the yield of the NA-deficient virus was limited, it formed large plaques when applied to MDCK cell cultures, indicating that the virus was able to spread to adjacent cells. Furthermore, the NA-deficient virus was eluted from chicken erythrocytes at 37 °C, even in the presence of the antiviral drug peramivir. Spread of this NA-deficient virus may pose a potential threat to anti-influenza therapies.

Journal

Archives of VirologySpringer Journals

Published: Apr 1, 2014

References

  • An update on swine-origin influenza virus A/H1N1: a review
    Schnitzler, SU; Schnitzler, P
  • Functional balance between haemagglutinin and neuraminidase in influenza virus infections
    Wagner, R; Matrosovich, M; Klenk, HD

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