An infectious full-length cDNA clone of potato virus Y NTN-NW , a recently reported strain of PVY that causes potato tuber necrotic ringspot disease

An infectious full-length cDNA clone of potato virus Y NTN-NW , a recently reported strain of PVY... A full-length infectious cDNA clone of potato virus Y (PVY) was constructed based on an isolate of the PVY NTN-NW strain, SYR-II-2-8, which is able to cause potato tuber necrotic ringspot disease (PTNRD). Silent point mutations were introduced to modify sequences similar to the prokaryotic promoter elements detected at the 5’ terminus of the P3 coding region of SYR-II-2-8. This, along with modification of the growing conditions of E. coli , led to the successful construction of a stable full-length infectious cDNA clone, named p2-8C3. The biological properties of p2-8C3 were identical to those of SYR-II-2-8, both of which induced PTNRD in potato, veinal necrosis in tobacco, and similar symptoms in the potato cultivars inoculated. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

An infectious full-length cDNA clone of potato virus Y NTN-NW , a recently reported strain of PVY that causes potato tuber necrotic ringspot disease

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Publisher
Springer Vienna
Copyright
Copyright © 2011 by Springer-Verlag
Subject
Biomedicine; Medical Microbiology; Virology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-011-1062-4
Publisher site
See Article on Publisher Site

Abstract

A full-length infectious cDNA clone of potato virus Y (PVY) was constructed based on an isolate of the PVY NTN-NW strain, SYR-II-2-8, which is able to cause potato tuber necrotic ringspot disease (PTNRD). Silent point mutations were introduced to modify sequences similar to the prokaryotic promoter elements detected at the 5’ terminus of the P3 coding region of SYR-II-2-8. This, along with modification of the growing conditions of E. coli , led to the successful construction of a stable full-length infectious cDNA clone, named p2-8C3. The biological properties of p2-8C3 were identical to those of SYR-II-2-8, both of which induced PTNRD in potato, veinal necrosis in tobacco, and similar symptoms in the potato cultivars inoculated.

Journal

Archives of VirologySpringer Journals

Published: Nov 1, 2011

References

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