An improved polyclonal antiserum for detecting Ryegrass mosaic rymovirus

An improved polyclonal antiserum for detecting Ryegrass mosaic rymovirus Ryegrass mosaic virus (RGMV) is considered the most serious and widespread virus infecting temperate pasture grasses. The use of visible symptoms to diagnose infection is unreliable and ELISA analysis requires antibodies with broad cross-reactivity. Here we describe the production of a polyclonal antiserum (PAb-cp3′Δ) using a bacterially expressed RGMV coat protein fragment. The PAb-cp3′Δ antiserum is specific for RGMV and recognises RGMV strains from each major phylogenetic cluster. PAb-cp3′Δ may be used in ELISAs for fast, accurate and inexpensive detection of RGMV. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

An improved polyclonal antiserum for detecting Ryegrass mosaic rymovirus

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Publisher
Springer-Verlag
Copyright
Copyright © 2005 by Springer-Verlag/Wien
Subject
Biomedicine; Medical Microbiology; Infectious Diseases; Virology
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-005-0531-z
Publisher site
See Article on Publisher Site

Abstract

Ryegrass mosaic virus (RGMV) is considered the most serious and widespread virus infecting temperate pasture grasses. The use of visible symptoms to diagnose infection is unreliable and ELISA analysis requires antibodies with broad cross-reactivity. Here we describe the production of a polyclonal antiserum (PAb-cp3′Δ) using a bacterially expressed RGMV coat protein fragment. The PAb-cp3′Δ antiserum is specific for RGMV and recognises RGMV strains from each major phylogenetic cluster. PAb-cp3′Δ may be used in ELISAs for fast, accurate and inexpensive detection of RGMV.

Journal

Archives of VirologySpringer Journals

Published: Sep 1, 2005

References

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