Indian J Microbiol https://doi.org/10.1007/s12088-018-0743-z ORIGINAL RESEARCH ARTICLE An Improved Method of Preparing High Efﬁciency Transformation Escherichia coli with Both Plasmids and Larger DNA Fragments 2 2 2 1 1 • • • • • Jingjing Liu Wenwen Chang Lei Pan Xiaoyun Liu Lufang Su 1 3 1 • • Weiying Zhang Qin Li Yu Zheng Received: 17 January 2018 / Accepted: 22 May 2018 Association of Microbiologists of India 2018 Abstract The high-throughput, cost-efﬁcient transforma- efﬁciency transformation experiments under normal labo- tion systems determine the success of gene cloning and ratory conditions. functional analysis. Among various factors that affect this transformation systems, the competence ability of target Keywords Biotransformation Competent cells High cells is one of the most important factors. We found transformation efﬁciency Molecular cloning antimicrobial peptides LFcin-B can increase the perme- ability of the cell membrane, and their lethal antibacterial properties can be inhibited by moderately high concentra- Introduction 2? 2? tions of Ca and Mn . In this study, we established a convenient and rapid method (CRM) by adding small Transformation involves introducing exogenous DNA into concentrations of (0.35 mg/L) and moderately high con- receptor bacteria to produce new genetic traits. It is
Indian Journal of Microbiology – Springer Journals
Published: May 29, 2018
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera