An Improved Method of Preparing High Efficiency Transformation Escherichia coli with Both Plasmids and Larger DNA Fragments

An Improved Method of Preparing High Efficiency Transformation Escherichia coli with Both... Indian J Microbiol https://doi.org/10.1007/s12088-018-0743-z ORIGINAL RESEARCH ARTICLE An Improved Method of Preparing High Efficiency Transformation Escherichia coli with Both Plasmids and Larger DNA Fragments 2 2 2 1 1 • • • • • Jingjing Liu Wenwen Chang Lei Pan Xiaoyun Liu Lufang Su 1 3 1 • • Weiying Zhang Qin Li Yu Zheng Received: 17 January 2018 / Accepted: 22 May 2018 Association of Microbiologists of India 2018 Abstract The high-throughput, cost-efficient transforma- efficiency transformation experiments under normal labo- tion systems determine the success of gene cloning and ratory conditions. functional analysis. Among various factors that affect this transformation systems, the competence ability of target Keywords Biotransformation  Competent cells  High cells is one of the most important factors. We found transformation efficiency  Molecular cloning antimicrobial peptides LFcin-B can increase the perme- ability of the cell membrane, and their lethal antibacterial properties can be inhibited by moderately high concentra- Introduction 2? 2? tions of Ca and Mn . In this study, we established a convenient and rapid method (CRM) by adding small Transformation involves introducing exogenous DNA into concentrations of (0.35 mg/L) and moderately high con- receptor bacteria to produce new genetic traits. It is http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Indian Journal of Microbiology Springer Journals

An Improved Method of Preparing High Efficiency Transformation Escherichia coli with Both Plasmids and Larger DNA Fragments

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Publisher
Springer India
Copyright
Copyright © 2018 by Association of Microbiologists of India
Subject
Life Sciences; Microbiology; Medical Microbiology
ISSN
0046-8991
eISSN
0973-7715
D.O.I.
10.1007/s12088-018-0743-z
Publisher site
See Article on Publisher Site

Abstract

Indian J Microbiol https://doi.org/10.1007/s12088-018-0743-z ORIGINAL RESEARCH ARTICLE An Improved Method of Preparing High Efficiency Transformation Escherichia coli with Both Plasmids and Larger DNA Fragments 2 2 2 1 1 • • • • • Jingjing Liu Wenwen Chang Lei Pan Xiaoyun Liu Lufang Su 1 3 1 • • Weiying Zhang Qin Li Yu Zheng Received: 17 January 2018 / Accepted: 22 May 2018 Association of Microbiologists of India 2018 Abstract The high-throughput, cost-efficient transforma- efficiency transformation experiments under normal labo- tion systems determine the success of gene cloning and ratory conditions. functional analysis. Among various factors that affect this transformation systems, the competence ability of target Keywords Biotransformation  Competent cells  High cells is one of the most important factors. We found transformation efficiency  Molecular cloning antimicrobial peptides LFcin-B can increase the perme- ability of the cell membrane, and their lethal antibacterial properties can be inhibited by moderately high concentra- Introduction 2? 2? tions of Ca and Mn . In this study, we established a convenient and rapid method (CRM) by adding small Transformation involves introducing exogenous DNA into concentrations of (0.35 mg/L) and moderately high con- receptor bacteria to produce new genetic traits. It is

Journal

Indian Journal of MicrobiologySpringer Journals

Published: May 29, 2018

References

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