An improved method for obtaining highly polymorphic microsatellite markers of increased length

An improved method for obtaining highly polymorphic microsatellite markers of increased length Mammalian Genome 9, 918–920 (1998). Incorporating Mouse Genome © Springer-Verlag New York Inc. 1998 An improved method for obtaining highly polymorphic microsatellite markers of increased length Oksana I. Dukhanina, Vladimir E. Sverdlov Department of Physiology and Molecular Medicine, Medical College of Ohio, 3035 Arlington Avenue, Toledo, Ohio 43614-5804, USA Received: 8 May 1998 / Accepted: 17 July 1998 While mammalian genome projects are well under development, through a 1% agarose gel, and the area of the gel containing DNA questions of efficient and optimal procedures leading toward dense ranging from 300 to 1300 bp was excised. The DNA was extracted genetic maps remain. Here we report a fast and simple procedure from the gel with GeneClean kit (Bio-101, Vista, Calif.), ligated of obtaining a small-insert genomic library strongly enriched for into PCR 2.1 vector (TA Cloning Kit, Invitrogen, San Diego, microsatellites with increased length. The method is based on the Calif.) and used for transformation of INVaF’ One Shot competent avidin-streptavidin system to capture microsatellites annealed to a cells, provided with the kit. The Chr 10-enriched library was pre- biotinylated probe and employs unique binding and washing con- pared according to the above described procedure except that the ditions. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Mammalian Genome Springer Journals

An improved method for obtaining highly polymorphic microsatellite markers of increased length

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Publisher
Springer-Verlag
Copyright
Copyright © 1998 by Springer-Verlag New York Inc.
Subject
Life Sciences; Cell Biology; Animal Genetics and Genomics; Human Genetics
ISSN
0938-8990
eISSN
1432-1777
D.O.I.
10.1007/s003359900898
Publisher site
See Article on Publisher Site

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