Mammalian Genome 9, 918–920 (1998). Incorporating Mouse Genome © Springer-Verlag New York Inc. 1998 An improved method for obtaining highly polymorphic microsatellite markers of increased length Oksana I. Dukhanina, Vladimir E. Sverdlov Department of Physiology and Molecular Medicine, Medical College of Ohio, 3035 Arlington Avenue, Toledo, Ohio 43614-5804, USA Received: 8 May 1998 / Accepted: 17 July 1998 While mammalian genome projects are well under development, through a 1% agarose gel, and the area of the gel containing DNA questions of efficient and optimal procedures leading toward dense ranging from 300 to 1300 bp was excised. The DNA was extracted genetic maps remain. Here we report a fast and simple procedure from the gel with GeneClean kit (Bio-101, Vista, Calif.), ligated of obtaining a small-insert genomic library strongly enriched for into PCR 2.1 vector (TA Cloning Kit, Invitrogen, San Diego, microsatellites with increased length. The method is based on the Calif.) and used for transformation of INVaF’ One Shot competent avidin-streptavidin system to capture microsatellites annealed to a cells, provided with the kit. The Chr 10-enriched library was pre- biotinylated probe and employs unique binding and washing con- pared according to the above described procedure except that the ditions.
Mammalian Genome – Springer Journals
Published: Nov 1, 1998
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera