An HDAC inhibitor increases Ac MNPV gene expression in mammalian cells

An HDAC inhibitor increases Ac MNPV gene expression in mammalian cells The baculovirus Autographa californica multiple nucleopolyhedrovirus ( Ac MNPV) is used as a safer viral vector in mammalian cells with potential applications in gene therapy. However, the mechanism for the insusceptibility of mammalian cells to proliferative infection by entomopathogenic viruses is not well understood. Here, we studied the significance of epigenetic modifications such as histone acetylation, histone methylation and HP1 accumulation for Ac MNPV gene expression in mammalian BHK cells. Real-time PCR and chromatin immunoprecipitation with sodium butyrate revealed an important relationship between viral gene expression and histone acetylation, with implications for a mechanism of suppression of Ac MNPV gene expression in BHK cells. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

An HDAC inhibitor increases Ac MNPV gene expression in mammalian cells

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Publisher
Springer Journals
Copyright
Copyright © 2010 by Springer-Verlag
Subject
Biomedicine; Infectious Diseases; Medical Microbiology ; Virology
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-010-0614-3
Publisher site
See Article on Publisher Site

Abstract

The baculovirus Autographa californica multiple nucleopolyhedrovirus ( Ac MNPV) is used as a safer viral vector in mammalian cells with potential applications in gene therapy. However, the mechanism for the insusceptibility of mammalian cells to proliferative infection by entomopathogenic viruses is not well understood. Here, we studied the significance of epigenetic modifications such as histone acetylation, histone methylation and HP1 accumulation for Ac MNPV gene expression in mammalian BHK cells. Real-time PCR and chromatin immunoprecipitation with sodium butyrate revealed an important relationship between viral gene expression and histone acetylation, with implications for a mechanism of suppression of Ac MNPV gene expression in BHK cells.

Journal

Archives of VirologySpringer Journals

Published: Apr 1, 2010

References

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