An expanded collection of mouse Y Chromosome RDA clones

An expanded collection of mouse Y Chromosome RDA clones Mammalian Genome 8, 510-512 (1997). 9 Springer-Verlag New York Inc. 1997 David E. Bergstrom, 1 Hailing Yan, 2 Manjula M. Sonti, 1 Sandya Narayanswami, 1 Janet K. Bayleran, 2 Elizabeth M. Simpson 1 1The Jackson Laboratory, 600 Main Street, Bar Harbor, Maine 04609, USA 2Eastern Maine Medical Center, 489 State Street, Bangor, Maine 04401, USA Received: 13 October 1996 / Accepted: 25 February 1997 If we are to understand the structure and function of the mamma- representations (Lisitsyn et al. 1993). Figure 1 shows the amplifi- lian genome and its transmission to subsequent generations, we cation products after the first and second rounds of RDA subtrac- cannot neglect the Y Chromosome (Chr). However, certain prop- tion. As can be seen, the first-round product is characterized by a erties of the Y have made it refractory to analysis. The bulk of the number of discrete bands above a background smear. The second- Y Chr does not recombine, and thus genetic maps based on stan- round product shows an enrichment of each of the discrete bands dard Mendelian recombination are not feasible. For this reason, and a reduction in the intensity of the background DNA. Previous investigators have relied on physical http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Mammalian Genome Springer Journals

An expanded collection of mouse Y Chromosome RDA clones

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Publisher
Springer-Verlag
Copyright
Copyright © 1997 by Springer-Verlag
Subject
Life Sciences; Cell Biology; Anatomy; Zoology
ISSN
0938-8990
eISSN
1432-1777
D.O.I.
10.1007/s003359900486
Publisher site
See Article on Publisher Site

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