Appl Biochem Biotechnol https://doi.org/10.1007/s12010-018-2785-4 An Enzyme Immunoassay for Determining Immunoreactive Trypsinogen (IRT) in Dried Blood Spots on Filter Paper Using an Ultra-Microanalytical System 1 2 Elisa M. Castells Martínez & Ernesto Carlos González & 3 1 1 Yileidis Tejeda & Amarilys Frómeta & Odalys Martín & 1 1 1 Maryeris Espinosa & Lesley del Río & Pedro L. Pérez & 4 1 5 Greilys Morejón & Yenitse Perea & Antonio Melchor Received: 19 February 2018 /Accepted: 15 May 2018 Springer Science+Business Media, LLC, part of Springer Nature 2018 Abstract Cystic fibrosis (CF) is a severe autosomal recessive disorder. It is caused by mutations in the CF transmembrane conductance regulator gene. Early diagnosis of CF can be carried out by determining high immunoreactive trypsinogen (IRT) blood values in newborns. A simple sandwich-type ultramicroELISA assay (UMELISA®) has been developed for the measurement of IRT in dried blood spots on filter paper. Strips coated with a high affinity monoclonal antibody directed against IRT are used as solid phase, to ensure the specificity of the assay. The assay is carried out within 20 h. The useful rank of the curve is 0–500 ng/mL, and the lowest detectable Highlights � IRT levels in newborns
Applied Biochemistry and Biotechnology – Springer Journals
Published: May 29, 2018
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