Biotechnol Lett (2018) 40:1049–1055 https://doi.org/10.1007/s10529-018-2569-5 ORIGINAL RESEARCH PAPER An efﬁcient biocatalytic synthesis of imidazole-4-acetic acid . . . . . Weirui Zhao Huanru Ding Sheng Hu Jun Huang Changjiang Lv . . . Jiaqi Mei Zhihua Jin Shanjing Yao Lehe Mei Received: 17 March 2018 / Accepted: 16 May 2018 / Published online: 23 May 2018 Springer Science+Business Media B.V., part of Springer Nature 2018 Abstract is not only environmentally friendly, but also more Objective To develop a new and efﬁcient biocat- cost effective, thus being promising for industrial IAA alytic synthesis method of imidazole-4-acetic acid production. (IAA) from L-histidine (L-His). Results L-His was converted to imidazole-4-pyruvic Keywords H O Imidazole-4-acetic acid L- 2 2 acid (IPA) by an Escherichia coli whole-cell biocat- Histidine Membrane-bound L-amino acid deaminase alyst expressing membrane-bound L-amino acid deaminase (mL-AAD) from Proteus vulgaris ﬁrstly. The obtained IPA was subsequently decarboxylated to IAA under the action of H O . Under optimum Introduction 2 2 conditions, 34.97 mM IAA can be produced from 50 mM L-His, with a yield of 69.9%. Imidazole-4-acetic acid (IAA) is one of the most Conclusions Compared to the traditional chemical important imidazole derivatives, naturally occurring synthesis, this
Biotechnology Letters – Springer Journals
Published: May 23, 2018
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