An assessment of molecular variability and recombination patterns in South African isolates of Potato virus Y

An assessment of molecular variability and recombination patterns in South African isolates of... The coat protein (CP) gene of 75 South African Potato virus Y (PVY) isolates was amplified using reverse-transcriptase polymerase chain reaction (RT-PCR). The resulting cDNA products were cloned and sequenced. These sequences were used to identify the strains to which the isolates belonged. Some, when compared to reference sequences, belonged to the PVY N and PVY O strains. A number of isolates were found to demonstrate significant homology to PVY N strains from China. A large number of South African isolates possessed CP sequences showing evidence of recombination between PVY N and PVY O strains, similar to those of PVY NTN isolates. Multiplex RT-PCR analysis allowed further differentiation of PVY O isolates and revealed that the majority were of the PVY N -Wilga strain. It was deduced that the most likely way in which these isolates reached South Africa was via the importation of infected material. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

An assessment of molecular variability and recombination patterns in South African isolates of Potato virus Y

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Publisher
Springer Vienna
Copyright
Copyright © 2009 by Springer-Verlag
Subject
Biomedicine; Infectious Diseases; Medical Microbiology ; Virology
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-009-0525-3
Publisher site
See Article on Publisher Site

Abstract

The coat protein (CP) gene of 75 South African Potato virus Y (PVY) isolates was amplified using reverse-transcriptase polymerase chain reaction (RT-PCR). The resulting cDNA products were cloned and sequenced. These sequences were used to identify the strains to which the isolates belonged. Some, when compared to reference sequences, belonged to the PVY N and PVY O strains. A number of isolates were found to demonstrate significant homology to PVY N strains from China. A large number of South African isolates possessed CP sequences showing evidence of recombination between PVY N and PVY O strains, similar to those of PVY NTN isolates. Multiplex RT-PCR analysis allowed further differentiation of PVY O isolates and revealed that the majority were of the PVY N -Wilga strain. It was deduced that the most likely way in which these isolates reached South Africa was via the importation of infected material.

Journal

Archives of VirologySpringer Journals

Published: Dec 1, 2009

References

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