An assessment of molecular variability and recombination patterns in South African isolates of Potato virus Y

An assessment of molecular variability and recombination patterns in South African isolates of... The coat protein (CP) gene of 75 South African Potato virus Y (PVY) isolates was amplified using reverse-transcriptase polymerase chain reaction (RT-PCR). The resulting cDNA products were cloned and sequenced. These sequences were used to identify the strains to which the isolates belonged. Some, when compared to reference sequences, belonged to the PVY N and PVY O strains. A number of isolates were found to demonstrate significant homology to PVY N strains from China. A large number of South African isolates possessed CP sequences showing evidence of recombination between PVY N and PVY O strains, similar to those of PVY NTN isolates. Multiplex RT-PCR analysis allowed further differentiation of PVY O isolates and revealed that the majority were of the PVY N -Wilga strain. It was deduced that the most likely way in which these isolates reached South Africa was via the importation of infected material. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

An assessment of molecular variability and recombination patterns in South African isolates of Potato virus Y

Loading next page...
 
/lp/springer_journal/an-assessment-of-molecular-variability-and-recombination-patterns-in-ZbsPrP8lVq
Publisher
Springer Vienna
Copyright
Copyright © 2009 by Springer-Verlag
Subject
Biomedicine; Infectious Diseases; Medical Microbiology ; Virology
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-009-0525-3
Publisher site
See Article on Publisher Site

Abstract

The coat protein (CP) gene of 75 South African Potato virus Y (PVY) isolates was amplified using reverse-transcriptase polymerase chain reaction (RT-PCR). The resulting cDNA products were cloned and sequenced. These sequences were used to identify the strains to which the isolates belonged. Some, when compared to reference sequences, belonged to the PVY N and PVY O strains. A number of isolates were found to demonstrate significant homology to PVY N strains from China. A large number of South African isolates possessed CP sequences showing evidence of recombination between PVY N and PVY O strains, similar to those of PVY NTN isolates. Multiplex RT-PCR analysis allowed further differentiation of PVY O isolates and revealed that the majority were of the PVY N -Wilga strain. It was deduced that the most likely way in which these isolates reached South Africa was via the importation of infected material.

Journal

Archives of VirologySpringer Journals

Published: Dec 1, 2009

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off