Mammalian Genome 8, 783–784 (1997). © Springer-Verlag New York Inc. 1997 fat An assay for the Cpe obesity mutation created with a modified primer Jerilyn A. Walker, Gary E. Truett Pennington Biomedical Research Center, 6400 Perkins Road, Baton Rouge, Louisiana 70808-4124, USA Received: 17 March 1997 / Accepted: 7 May 1997 fat Cpe is an autosomal recessive mutation that causes adult-onset GTCTCCTCCGTGCAGATTGGCCG38, where the altered base obesity (Coleman and Eicher 1990). The mutation arose in the is underlined. The forward primer (CpeF) was selected by Oligo HRS mouse strain and was transferred to C57BLKS for compari- 5.0 to optimize amplification of the target sequence. The sequence son with two other obesity mutations, diabetes (db) and obese (ob), of primer CpeF is 58 CAGCTTGCCCCCGAGACCAAGG 38. which also cause overt diabetes mellitus on the C57BLKS back- The DNA predicted to be amplified by primers CpeF and CpeR ground (Coleman and Eicher 1990; Naggert et al. 1995). is 90 base pairs long. Mutant DNA is predicted to cut with MspI fat fat C57BLKS-Cpe /Cpe mutants differ from C57BLKS-db/db and to generate 64 and 26 bp fragments, and wild-type DNA is pre- C57BLKS-ob/ob mice in several ways. While db/db and ob/ob dicted to
Mammalian Genome – Springer Journals
Published: Oct 1, 1997
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