An aptamer based sulfadimethoxine assay that uses magnetized upconversion nanoparticles

An aptamer based sulfadimethoxine assay that uses magnetized upconversion nanoparticles The authors describe an aptamer based method for the determination of the antibiotic sulfadimethoxine (SLF). It is making use of upconversion nanoparticles (UCNPs) that act as an internal fluorophors signals, and of aptamers that can specifically recognize SLF. The UCNPs were deposited on the surface of magnetic nanoparticles (magNPs) functionalized with the aptamer. In the presence of SLF, parts of the UCNPs dissociate from the surface of the magNPs, and this results in decreased fluorescence intensity. The findings were exploited to design a fluorometric assay for SLF that has a 0.11 ng·mL−1 detection limit. The method was successfully applied to the quantification of SLF in spiked samples of perch and catfish. Recoveries range from 80.3% to 92.7%, and RSDs from 2.4% to 3.6%. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Microchimica Acta Springer Journals

An aptamer based sulfadimethoxine assay that uses magnetized upconversion nanoparticles

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Publisher
Springer Vienna
Copyright
Copyright © 2017 by Springer-Verlag GmbH Austria
Subject
Chemistry; Nanochemistry; Nanotechnology; Characterization and Evaluation of Materials; Analytical Chemistry; Microengineering
ISSN
0026-3672
eISSN
1436-5073
D.O.I.
10.1007/s00604-017-2378-x
Publisher site
See Article on Publisher Site

Abstract

The authors describe an aptamer based method for the determination of the antibiotic sulfadimethoxine (SLF). It is making use of upconversion nanoparticles (UCNPs) that act as an internal fluorophors signals, and of aptamers that can specifically recognize SLF. The UCNPs were deposited on the surface of magnetic nanoparticles (magNPs) functionalized with the aptamer. In the presence of SLF, parts of the UCNPs dissociate from the surface of the magNPs, and this results in decreased fluorescence intensity. The findings were exploited to design a fluorometric assay for SLF that has a 0.11 ng·mL−1 detection limit. The method was successfully applied to the quantification of SLF in spiked samples of perch and catfish. Recoveries range from 80.3% to 92.7%, and RSDs from 2.4% to 3.6%.

Journal

Microchimica ActaSpringer Journals

Published: Jun 27, 2017

References

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