Amplification and analysis of DNA flanking known sequences of a novel herpesvirus from green turtles with fibropapilloma

Amplification and analysis of DNA flanking known sequences of a novel herpesvirus from green... A 1,632-bp fragment, flanking the original 483-bp region of the DNA polymerase gene of a novel herpesvirus found in tissues of green turtles ( Chelonia mydas ) with fibropapilloma, was amplified from the circularized Eco RI-cut DNA extracted from tumor tissues by inverse PCR. The resultant 2,019-bp partial sequence of the DNA polymerase gene of the newfound herpesvirus, including the original 483-bp region, showed a high degree of homology at both the nucleotide and amino acid levels with that of other human and animal herpesviruses. Phylogenetic analysis confirmed that this novel herpesvirus belonged to the Alphaherpesvirinae subfamily. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Amplification and analysis of DNA flanking known sequences of a novel herpesvirus from green turtles with fibropapilloma

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Publisher
Springer-Verlag
Copyright
Copyright © 2000 by Springer-Verlag/Wien
Subject
Legacy
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s007050070015
Publisher site
See Article on Publisher Site

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