Amperometric immunoassay for the tumor marker neuron-specific enolase using a glassy carbon electrode modified with a nanocomposite consisting of polyresorcinol and of gold and platinum nanoparticles

Amperometric immunoassay for the tumor marker neuron-specific enolase using a glassy carbon... A glassy carbon electrode was modified with a redox-active nanocomposite consisting of polyresorcinol, gold nanoparticles (NPs) and platinum NPs. This nanocomposite possesses the following outstanding advantages: (1) strong electrochemical signal towards H2O2 at 0.92 V (vs Ag/AgCl); (2) good conductivity; and (3) ease of immobilization of antibody without the use of coupling agents. Based on these properties, a label-free amperometric immunoassay for the tumor marker neuron-specific enolase (NSE) was developed. The immunoelectrode has a linear response in the 10 pg·mL−1 to 100 ng·mL−1 NSE concentration range and a 7.8 pg·mL−1 detection limit (at an SNR of 3). The method was applied to the quantitation of NSE in human serum samples and showed satisfactory accuracy compared to an electrochemiluminescence immunoassay, a commonly used method in clinical applications. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Microchimica Acta Springer Journals

Amperometric immunoassay for the tumor marker neuron-specific enolase using a glassy carbon electrode modified with a nanocomposite consisting of polyresorcinol and of gold and platinum nanoparticles

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Publisher
Springer Vienna
Copyright
Copyright © 2017 by Springer-Verlag Wien
Subject
Chemistry; Nanochemistry; Nanotechnology; Characterization and Evaluation of Materials; Analytical Chemistry; Microengineering
ISSN
0026-3672
eISSN
1436-5073
D.O.I.
10.1007/s00604-017-2287-z
Publisher site
See Article on Publisher Site

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