Amperometric determination of organophosphate pesticides using a acetylcholinesterase based biosensor made from nitrogen-doped porous carbon deposited on a boron-doped diamond electrode

Amperometric determination of organophosphate pesticides using a acetylcholinesterase based... The authors describe an amperometric biosensor for the determination of organophosphate pesticides (OPs) via inhibition of the enzyme acetylcholinesterase (AChE). The enzyme was immobilized on nitrogen-doped porous carbon and then placed on boron-doped diamond (BDD). The Michaelis-Menten constant for immobilized AChE is 0.177 mmol L−1, indicating that AChE has a stronger enzymatic activity and affinity due to the introduction of nitrogen-doped porous carbon. The biosensor was applied to the determination of the OPs dichlorvos and fenitrothion. The efficiency of the inhibition by dichlorvos increases linearly in the 100 pg·L-1 to 10.0 μg·L-1 concentration range. The detection limit is as low as 1.50 pg·L-1. The inhibition by fenitrothion can be detected in the same concentration range, and the detection limit is 4.40 pg·L-1 (calculated for 10% inhibition). The unique sensitivity of this assay make it a most attractive tool for the detection of OPs. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Microchimica Acta Springer Journals

Amperometric determination of organophosphate pesticides using a acetylcholinesterase based biosensor made from nitrogen-doped porous carbon deposited on a boron-doped diamond electrode

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Publisher
Springer Vienna
Copyright
Copyright © 2017 by Springer-Verlag GmbH Austria
Subject
Chemistry; Nanochemistry; Nanotechnology; Characterization and Evaluation of Materials; Analytical Chemistry; Microengineering
ISSN
0026-3672
eISSN
1436-5073
D.O.I.
10.1007/s00604-017-2380-3
Publisher site
See Article on Publisher Site

Abstract

The authors describe an amperometric biosensor for the determination of organophosphate pesticides (OPs) via inhibition of the enzyme acetylcholinesterase (AChE). The enzyme was immobilized on nitrogen-doped porous carbon and then placed on boron-doped diamond (BDD). The Michaelis-Menten constant for immobilized AChE is 0.177 mmol L−1, indicating that AChE has a stronger enzymatic activity and affinity due to the introduction of nitrogen-doped porous carbon. The biosensor was applied to the determination of the OPs dichlorvos and fenitrothion. The efficiency of the inhibition by dichlorvos increases linearly in the 100 pg·L-1 to 10.0 μg·L-1 concentration range. The detection limit is as low as 1.50 pg·L-1. The inhibition by fenitrothion can be detected in the same concentration range, and the detection limit is 4.40 pg·L-1 (calculated for 10% inhibition). The unique sensitivity of this assay make it a most attractive tool for the detection of OPs.

Journal

Microchimica ActaSpringer Journals

Published: Jun 21, 2017

References

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