Mammalian Genome 10, 423–427 (1999). Incorporating Mouse Genome © Springer-Verlag New York Inc. 1999 Alternative splicing of hMSH4: two isoforms in testis and abnormal transcripts in somatic tissues 1 1 2 1 1 1 S. Santucci-Darmanin, R. Paul, J.F. Michiels, A. Saunie `res, C. Desnuelle, V. Paquis-Flucklinger Laboratoire de Neurobiologie Cellulaire, UMR 6549 CNRS/UNSA, Faculte´de Me ´decine, Av. de Valombrose, 06107 Nice cedex 2, France Laboratoire d’Anatomie Pathologique, Faculte´de Me ´decine, Av. de Valombrose, 06107 Nice cedex 2, France Received: 31 August 1998 / Accepted: 15 December 1998 The Escherichia coli MutHLS mismatch repair pathway has been Flucklinger et al. 1997). We next analyzed the expression of conserved throughout evolution. A large body of work has led to hMSH4 in human testis by RT-PCR. Reverse transcription reac- study of the respective roles of eukaryotic MSH and MLH genes in tion was followed by a two-step PCR procedure that allowed am- somatic DNA repair (Fishel and Wilson 1997). If it is now clear plification of the total cDNA coding region. PCR products were that some of these genes are also involved in meiosis, less is directly cloned into the pGEM-T vector (Promega, Madison, WI). known about their functions. In Saccharomyces
Mammalian Genome – Springer Journals
Published: Apr 1, 1999
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