Alterations of histone modifications at the senescence-associated gene HvS40 in barley during senescence

Alterations of histone modifications at the senescence-associated gene HvS40 in barley during... The barley gene HvS40, encoding a putative regulator of leaf senescence, is strongly induced during leaf senescence. As shown by chromatin immunoprecipitation, euchromatic histone modification H3K9ac is added at promoter close to ATG and coding sequence of HvS40 after onset of senescence. In parallel, level of heterochromatic H3K9me2 decreases at this gene. Bisulfite sequencing revealed no DNA-methylation in this region, but a heavily methylated DNA-island, starting 664 bp upstream from translational start site in both, mature and senescent leaves. A decrease in DNA methylation in senescing leaves could be shown at one specific CpG motif at the end of this methylation island. In addition, global changes in chromatin structure during senescence were analyzed via immunocytology, revealing senescence-associated changes in spatial distribution of heterochromatic H3K9me2 patterns in the nuclei. Our results prove a senescence-specific mechanism, altering histone modification marks at distinct sequences of the senescence-associated gene HvS40 and altering distribution of heterochromatic areas in the nuclei. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

Alterations of histone modifications at the senescence-associated gene HvS40 in barley during senescence

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Publisher
Springer Netherlands
Copyright
Copyright © 2015 by Springer Science+Business Media Dordrecht
Subject
Life Sciences; Plant Sciences; Biochemistry, general; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1007/s11103-015-0358-2
Publisher site
See Article on Publisher Site

Abstract

The barley gene HvS40, encoding a putative regulator of leaf senescence, is strongly induced during leaf senescence. As shown by chromatin immunoprecipitation, euchromatic histone modification H3K9ac is added at promoter close to ATG and coding sequence of HvS40 after onset of senescence. In parallel, level of heterochromatic H3K9me2 decreases at this gene. Bisulfite sequencing revealed no DNA-methylation in this region, but a heavily methylated DNA-island, starting 664 bp upstream from translational start site in both, mature and senescent leaves. A decrease in DNA methylation in senescing leaves could be shown at one specific CpG motif at the end of this methylation island. In addition, global changes in chromatin structure during senescence were analyzed via immunocytology, revealing senescence-associated changes in spatial distribution of heterochromatic H3K9me2 patterns in the nuclei. Our results prove a senescence-specific mechanism, altering histone modification marks at distinct sequences of the senescence-associated gene HvS40 and altering distribution of heterochromatic areas in the nuclei.

Journal

Plant Molecular BiologySpringer Journals

Published: Aug 7, 2015

References

  • Epigenetic programming via histone methylation at WRKY53 controls leaf senescence in Arabidopsis thaliana
    Ay, N; Irmler, K; Fischer, A; Uhlemann, R; Reuter, G; Humbeck, K
  • Regulatory factors of leaf senescence are affected in Arabidopsis plants overexpressing the histone methyltransferase SUVH2
    Ay, N; Raum, U; Balazadeh, S; Seidensticker, T; Fischer, A; Reuter, G; Humbeck, K
  • Transcription factors regulating leaf senescence in Arabidopsis thaliana
    Balazadeh, S; Riano-Pachón, DM; Mueller-Roeber, B
  • Chromatin dynamics and Arabidopsis development
    Berger, F; Gaudin, V
  • High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
    Breeze, E; Harrison, E; McHattie, S
  • Comparative transcriptome analysis reveals significant differences in gene expression and signaling pathways between developmental and dark/starvation induced senescence in Arabidopsis
    Buchanan-Wollaston, V; Page, T; Harrison, E
  • Dynamic landscapes of four histone modifications during deetiolation in Arabidopsis
    Charron, JBF; He, H; Elling, AA; Denga, XW
  • Shotgun bisulphite sequencing of the Arabidopsis genome reveals DNA methylation patterning
    Cokus, SJ; Feng, S; Zhang, X; Chen, Z; Merriman, B; Haudenschild, CD; Pradhan, S; Nelson, SF; Pellegrini, M; Jacobsen, SE
  • Non-Rabl patterns of centromere and telomere distribution in the interphase nuclei of plant cells
    Dong, F; Jiang, J

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