Alterations in glutathione pool and some related enzymes in leaves and roots of pea plants treated with the herbicide glyphosate

Alterations in glutathione pool and some related enzymes in leaves and roots of pea plants... Our previous studies have demonstrated that application of glyphosate caused oxidative events in young pea and wheat plants. In this work, the changes in the endogenous level of glutathione (total and oxidized) and the activities of glutathione reductase (GR) and glutathione S-transferase (GST) after treatment with glyphosate were studied in pea plants (Pisum sativum L., cv. Skinado). Glyphosate was applied in two ways: (1) by leaf spraying with 10 mM solution; and (2) in nutrient medium as 0.01 mM solution. Measurements were made in both leaves and roots. Root and leaf treatments provoked the increase in both total and oxidized glutathione contents. Both types of herbicide application caused activation of GR in treated organs. Slight increase was detected also in untreated roots. It was found that glyphosate application to leaves provoked strong enhancement in the GST activity in leaves, while its root application stimulated the enzyme activity in the roots. We observed the higher GST activity in the organ directly treated with herbicide. Furthermore, we suggested that the activated isoforms of GST(s) participated in detoxification of hydrogen peroxide and lipid peroxides. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Alterations in glutathione pool and some related enzymes in leaves and roots of pea plants treated with the herbicide glyphosate

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Publisher
SP MAIK Nauka/Interperiodica
Copyright
Copyright © 2010 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Plant Sciences ; Plant Physiology
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443710010188
Publisher site
See Article on Publisher Site

Abstract

Our previous studies have demonstrated that application of glyphosate caused oxidative events in young pea and wheat plants. In this work, the changes in the endogenous level of glutathione (total and oxidized) and the activities of glutathione reductase (GR) and glutathione S-transferase (GST) after treatment with glyphosate were studied in pea plants (Pisum sativum L., cv. Skinado). Glyphosate was applied in two ways: (1) by leaf spraying with 10 mM solution; and (2) in nutrient medium as 0.01 mM solution. Measurements were made in both leaves and roots. Root and leaf treatments provoked the increase in both total and oxidized glutathione contents. Both types of herbicide application caused activation of GR in treated organs. Slight increase was detected also in untreated roots. It was found that glyphosate application to leaves provoked strong enhancement in the GST activity in leaves, while its root application stimulated the enzyme activity in the roots. We observed the higher GST activity in the organ directly treated with herbicide. Furthermore, we suggested that the activated isoforms of GST(s) participated in detoxification of hydrogen peroxide and lipid peroxides.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Jan 12, 2010

References

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