Alteration of cell responses to PrP Sc in prolonged cell culture and its effect on transmission of PrP Sc to neural cells

Alteration of cell responses to PrP Sc in prolonged cell culture and its effect on transmission... The mechanisms and processes of the uptake, intracellular trafficking and intercellular spread of PrP Sc and its transfer to neural cells are not clearly defined. The involvement of immune, intestinal, mast or peripheral neural cells in this process also remains unclear. The role of these cell types in the accumulation and transfer of PrP Sc to neural cells was investigated following short and prolonged exposure to the Chandler and Obihiro strains of scrapie PrP Sc for up to 28 days. Eight cell lines of murine immune, neural, intestinal and fibroblast cell types were tested. After transient degradation phases, certain immune, intestinal and neural cells accumulated PrP Sc for up to 28 days postinfection. When co-cultured with N2a-3/EGFP neuroblastoma cells for 4 days followed by several passages, the immune, intestinal and the neural cell lines were able to transfer infection to neural cells. Our results suggest that some of these cell types may have a role in PrP Sc accumulation and intercellular spread of PrP Sc infection to neural cells in vivo . http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Alteration of cell responses to PrP Sc in prolonged cell culture and its effect on transmission of PrP Sc to neural cells

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Publisher
Springer Vienna
Copyright
Copyright © 2013 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-012-1540-3
Publisher site
See Article on Publisher Site

Abstract

The mechanisms and processes of the uptake, intracellular trafficking and intercellular spread of PrP Sc and its transfer to neural cells are not clearly defined. The involvement of immune, intestinal, mast or peripheral neural cells in this process also remains unclear. The role of these cell types in the accumulation and transfer of PrP Sc to neural cells was investigated following short and prolonged exposure to the Chandler and Obihiro strains of scrapie PrP Sc for up to 28 days. Eight cell lines of murine immune, neural, intestinal and fibroblast cell types were tested. After transient degradation phases, certain immune, intestinal and neural cells accumulated PrP Sc for up to 28 days postinfection. When co-cultured with N2a-3/EGFP neuroblastoma cells for 4 days followed by several passages, the immune, intestinal and the neural cell lines were able to transfer infection to neural cells. Our results suggest that some of these cell types may have a role in PrP Sc accumulation and intercellular spread of PrP Sc infection to neural cells in vivo .

Journal

Archives of VirologySpringer Journals

Published: Mar 1, 2013

References

  • Characterization of the role of dendritic cells in prion transfer to primary neurons
    Langevin, C; Gousset, K; Costanzo, M; Richard-Le Goff, O; Zurzolo, C
  • Prion propagation in cultured cells
    Solassol, J; Crozet, C; Lehmann, S
  • Spreading of prions from the immune to the peripheral nervous system: a potential implication of dendritic cells
    Dorban, G; Defaweux, V; Heinen, E; Antoine, N
  • Misfolded PrP impairs the UPS by interaction with the 20S proteasome and inhibition of substrate entry
    Deriziotis, P; André, R; Smith, DM; Goold, R; Kinghorn, KJ; Kristiansen, M; Nathan, JA; Rosenzweig, R; Krutauz, D; Glickman, MH; Collinge, J; Goldberg, AL; Tabrizi, SJ
  • Prions hijack tunnelling nanotubes for intercellular spread
    Gousset, K; Schiff, E; Langevin, C; Marijanovic, Z; Caputo, A; Browman, DT; Chenouard, N; Chaumont, F; Martino, A; Enninga, J; Olivo-Marin, J-C; Maennel, D; Zurzolo, C

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