Reviews in Fish Biology and Fisheries 10: 281–292, 2001.
© 2001 Kluwer Academic Publishers. Printed in the Netherlands.
Allozymes, mtDNA and microsatellites study introgression in a stocked
trout population in France
& F. Bonhomme
Laboratoire d’Ethologie Exp´erimentale et Compar´ee, Universit´e Paris XIII 93 430 Villetaneuse Cedex, France
Laboratoire G´enome, Populations, Interactions, CNRS-UMR
5000, Universit´e Montpellier II, Cc63, Place Eug`ene Bataillon, 34 095 Montpellier Cedex 05, France
Accepted 22 October 2000
Abstract page 281
Materials and methods 282
Site location and sampling
Allozymes and mtDNA data
What is the explanation for the low variability in the Fontaine-de-Vaucluse sample?
The genetic impact of restocking
Lack of concordance among protein-coding and microsatellite loci
What information can we draw from our markers as an aid for the management of Mediterranean trout stocks?
Key words: bottleneck, brown trout, introgression, microsatellite, polymorphism, Salmo trutta
A comparative study of disturbed (downstream) and undisturbed (upstream) populations in a French Mediterranean
river (the Sorgue) was conducted to assess the fate of introgressing hatchery-genes in the aboriginal gene pool.
Variation was assessed at 27 protein-coding loci, three microsatellite loci and two mtDNA restriction sites.
The results were compared to those from another Mediterranean drainage basin (that of the river Orb). The
genetic pattern observed among markers and drainage basins was found to be noticeably different. A reduction
in population size is hypothesized to explain the lower variability observed in the upper part of the river
Sorgue. Estimations of hatchery gene ﬂow were different between loci but in the same range of values between