Mammalian Genome 8, 857-858 (1997). e//o///e 9 Springer-Verlag New York Inc. 1997 Allele-specific PCR assays for the tub and cpd at mutations Terry Maddatu, Jiirgen K. Naggert The Jackson Laboratory, 600 Main St., Bar Harbor, Maine 04609, USA Received: 25 May 1997 / Accepted: 1 July 1997 cpe e't (fat) and tub are mouse mutations that were originally noted allele-specific PCR assay was developed by using the mutageni- for adult onset obesity that is recognizable in C57BLKS-fat/fat cally separated PCR (MS-PCR) method described by Rust and mice between 10 and 14 weeks of age and in C57BL6-tub/tub coworkers (1993). The method relies on the competitive amplifi- between 16 and 20 weeks of age (Coleman and Eicher 1990). The cation between two allele-specific primers of differing lengths and earliest phenotype observable in fat is hyperinsulinemia (Coleman a common nonselective complementary strand primer. A deliber- and Eicher 1990) and in tub, an abnormal electroretinogram and ate mismatch is introduced in the sequence of the shorter selective auditory brainstem response at 4 weeks of age (Heckenlively et al. primer in one of the three bases next to the 3' terminal base. In the 1995; Ohlemiller et al. 1995). same region the longer
Mammalian Genome – Springer Journals
Published: Mar 24, 2009
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