Agrobacterium tumefaciens was used to transform fiber flax with the pBITUBA8 plasmid carrying the mutant α-tubulin gene imparting resistance to dinitroaniline herbicides and the nptII selective marker gene imparting resistance to kanamycin. The transformants were selected in parallel on media containing kanamycin and trifluralin (a dinitroaniline herbicide). The transgenic nature of the resultant regenerants resistant to dinitroaniline herbicides was confirmed by means of Southern blotting and polymerase chain reaction (PCR) analysis using specific probes for the ntpII gene and the gene of α-tubulin.
Russian Journal of Genetics – Springer Journals
Published: Oct 11, 2009
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