Agrobacterium-mediated transformation of flax with a mutant tubulin gene responsible for resistance to dinitroaniline herbicides

Agrobacterium-mediated transformation of flax with a mutant tubulin gene responsible for... Agrobacterium tumefaciens was used to transform fiber flax with the pBITUBA8 plasmid carrying the mutant α-tubulin gene imparting resistance to dinitroaniline herbicides and the nptII selective marker gene imparting resistance to kanamycin. The transformants were selected in parallel on media containing kanamycin and trifluralin (a dinitroaniline herbicide). The transgenic nature of the resultant regenerants resistant to dinitroaniline herbicides was confirmed by means of Southern blotting and polymerase chain reaction (PCR) analysis using specific probes for the ntpII gene and the gene of α-tubulin. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Genetics Springer Journals

Agrobacterium-mediated transformation of flax with a mutant tubulin gene responsible for resistance to dinitroaniline herbicides

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Publisher
Springer Journals
Copyright
Copyright © 2009 by Pleiades Publishing, Ltd.
Subject
Biomedicine; Microbial Genetics and Genomics; Animal Genetics and Genomics; Human Genetics
ISSN
1022-7954
eISSN
1608-3369
D.O.I.
10.1134/S1022795409100093
Publisher site
See Article on Publisher Site

Abstract

Agrobacterium tumefaciens was used to transform fiber flax with the pBITUBA8 plasmid carrying the mutant α-tubulin gene imparting resistance to dinitroaniline herbicides and the nptII selective marker gene imparting resistance to kanamycin. The transformants were selected in parallel on media containing kanamycin and trifluralin (a dinitroaniline herbicide). The transgenic nature of the resultant regenerants resistant to dinitroaniline herbicides was confirmed by means of Southern blotting and polymerase chain reaction (PCR) analysis using specific probes for the ntpII gene and the gene of α-tubulin.

Journal

Russian Journal of GeneticsSpringer Journals

Published: Oct 11, 2009

References

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