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E.J. Richards (1997)
Short Protocols in Molecular Biology
Hungtu Ma, M. Gu, George Liang (2004)
Plant regeneration from cultured immature embryos of Sorghum bicolor (L.) MoenchTheoretical and Applied Genetics, 73
H. Kaeppler, J. Pedersen (2004)
Evaluation of 41 elite and exotic inbred Sorghum genotypes for high quality callus productionPlant Cell, Tissue and Organ Culture, 48
S. Ohta, S. Mita, T. Hattori, K. Nakamura (1990)
Construction and Expression in Tobacco of a β-Glucuronidase (GUS) Reporter Gene Containing an Intron Within the Coding SequencePlant and Cell Physiology, 31
A. Perl, O. Lotan, Mohamad Abu-Abied, D. Holland (1996)
Establishment of an Agrobacterium-mediated transformation system for grape (Vitis vinifera L.): The role of antioxidants during grape–Agrobacterium interactionsNature Biotechnology, 14
M.T. Chan, T.M. Lee, H.H. Chang (1992)
Transformation of indica rice (Oryza sativa L.) mediated by AgrobacteriumPlant Cell Physiol., 33
A. Casas, A. Kononowicz, Theresa Haan, Lanying Zhang, D. Tomes, R. Bressan, P. Hasegawa (1997)
Transgenic sorghum plants obtained after microprojectile bombardment of immature inflorescencesIn Vitro Cellular & Developmental Biology - Plant, 33
M. Cho, Wen Jiang, P. Lemaux (1998)
Transformation of recalcitrant barley cultivars through improvement of regenerability and decreased albinismPlant Science, 138
(1997)
Design and Analysis of Experiments, 4th ed
T. Komari, Y. Hiei, Yasuhito Saito, Nobuhiko Murai, T. Kumashiro (1996)
Vectors carrying two separate T-DNAs for co-transformation of higher plants mediated by Agrobacterium tumefaciens and segregation of transformants free from selection markers.The Plant journal : for cell and molecular biology, 10 1
T. Nagai, K. Ibata, E. Park, Mie Kubota, K. Mikoshiba, A. Miyawaki (2002)
A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applicationsNature Biotechnology, 20
T. Cai, L. Butler (1990)
Plant regeneration from embryogenic callus initiated from immature inflorescences of several high-tannin sorghumsPlant Cell, Tissue and Organ Culture, 20
Y. Hiei, S. Ohta, T. Komari, T. Kumashiro (1994)
Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA.The Plant journal : for cell and molecular biology, 6 2
T. Murashige, F. Skoog (1962)
A revised medium for rapid growth and bio assays with tobacco tissue culturesPhysiologia Plantarum, 15
Karen Pollock, D. Barfield, R. Shields (1983)
The toxicity of antibiotics to plant cell culturesPlant Cell Reports, 2
M. Kramer, D. Coen (2006)
Enzymatic Amplification of DNA by PCR: Standard Procedures and OptimizationCurrent Protocols in Cytometry, 37
R. Fraley, S. Rogers, R. Horsch, P. Sanders, J. Flick, S. Adams, M. Bittner, L. Brand, C. Fink, J. Fry, G. Galluppi, S. Goldberg, N. Hoffmann, S. Woo (1983)
Expression of bacterial genes in plant cells.Proceedings of the National Academy of Sciences of the United States of America, 80 15
A. Christensen, R. Sharrock, P. Quail (1992)
Maize polyubiquitin genes: structure, thermal perturbation of expression and transcript splicing, and promoter activity following transfer to protoplasts by electroporationPlant Molecular Biology, 18
T. Aronen (1997)
Interactions between Agrobacterium tumefaciens and coniferous defence compounds α‐pinene and trans‐stilbeneForest Pathology, 27
X. Pu, R. Goodman (1992)
Induction of necrogenesis by Agrobacterium tumefaciens on grape explantsPhysiological and Molecular Plant Pathology, 41
(1998)
Molecular analysis of T0 plants
M. Battraw, Timothy Hall (1991)
Stable transformation of Sorghum bicolor protoplasts with chimeric neomycin phosphotransferase II and β-glucuronidase genesTheoretical and Applied Genetics, 82
G. Hansen (2000)
Evidence for Agrobacterium-induced apoptosis in maize cells.Molecular plant-microbe interactions : MPMI, 13 6
H. Fischbach, J. Levine (1953)
The identification of the antibiotics.Antibiotics & chemotherapy, 3 11
H. Zhu, S. Muthukrishana, S. Krishnaveni, G. Wilde, J.-M. Jeoung, G.H. Liang (1998)
Biolistic transformation of sorghum using a rice chitinase geneJ. Genet. Breed., 52
Ana Casas, A. Kononowicz, U. Zehr, D. Tomes, J. Axtell, Larry Butler, R. Bressan, P. Hasegawa (1993)
Transgenic sorghum plants via microprojectile bombardment.Proceedings of the National Academy of Sciences of the United States of America, 90 23
G. Lazo, P. Stein, R. Ludwig (1991)
A DNA Transformation–Competent Arabidopsis Genomic Library in AgrobacteriumBio/Technology, 9
M. Chan, Tse-Min Lee, Hsin-Hsiung Chang (1992)
Transformation of Indica rice (Oryza sativa L.) mediated by Agrobacterium tumefaciensPlant and Cell Physiology, 33
Gynheung An, Amitava Mitra, Hong Choi, Michael Costa, Kyungsook An, Robed Thornburg, Clarence Ryan (1989)
Functional analysis of the 3' control region of the potato wound-inducible proteinase inhibitor II gene.The Plant cell, 1 1
J. Miles, J. Guest (1984)
Nucleotide sequence and transcriptional start point of the phosphomannose isomerase gene (manA) of Escherichia coli.Gene, 32 1-2
P. Holford, H. Newbury (1992)
The effects of antibiotics and their breakdown products on the in vitro growth of Antirrhinum majusPlant Cell Reports, 11
D. Mccabe, W. Swain, B. Martinell, P. Christou (1988)
Stable Transformation of Soybean (Glycine Max) by Particle AccelerationBio/Technology, 6
C. Thompson, N. Movva, R. Tizard, R. Crameri, J. Davies, M. Lauwereys, J. Botterman (1987)
Characterization of the herbicide‐resistance gene bar from Streptomyces hygroscopicusThe EMBO Journal, 6
T. Cai, Barbara Daly, L. Butler (1987)
Callus induction and plant regeneration from shoot portions of mature embryos of high tannin sorghumsPlant Cell, Tissue and Organ Culture, 9
P. Bregitzer, R. Campbell, L. Dahleen, P. Lemaux, M. Cho (2000)
Development of transformation systems for elite barley cultivars., 30
R. Henry, J. Ronalds (2012)
Improvement of Cereal Quality by Genetic Engineering
C. Labarca, K. Paigen (1980)
A simple, rapid, and sensitive DNA assay procedure.Analytical biochemistry, 102 2
N.J. Shackelford, C.A. Chlan (1996)
Identification of antibiotics that are effective in eliminating Agrobacterium tumefaciensPlant Mol. Biol. Rep., 14
M. Abedinia, R. Henry, A. Blakeney, L. Lewin (1997)
Efficient transformation of rice
S. Tingay, D. Mcelroy, R. Kalla, Sarah Fieg, Ming-Bo Wang, S. Thornton, Richard Brettell (1997)
Agrobacterium tumefaciens‐mediated barley transformationPlant Journal, 11
Z.-Y. Zhao, W. Gu, T. Cai, L.A. Tagliani, D. Hondred, D. Bond, S. Krell, M.L. Rudert, W.B. Bruce, D.A. Pierce (1998)
Molecular analysis of T0 plants transformed by Agrobacterium and comparison of Agrobacterium-mediated transformation with bombardment transformation in maizeMaize Genet. Coop. Newsl., 72
T. Hagio, A. Blowers, E. Earle (1991)
Stable transformation of sorghum cell cultures after bombardment with DNA-coated microprojectilesPlant Cell Reports, 10
(1997)
Preparation of plant DNA using CTAB
A. Feinberg, B. Vogelstein (1983)
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.Analytical biochemistry, 132 1
H. Zhu, J. Jeoung, G. Liang, S. Muthukrishnan, S. Krishnaveni, G. Wilde (1998)
Biolistic transformation of sorghum using a rice chitinase gene [Sorghum bicolor (L.) Moench - Oryza sativa L.]Journal of Genetics and Breeding
C. Chu, Ching-Chu Wang, C. Sun, C. Hsu, K. Yin, Chih--Yin Chu, Fengqin Bi (1975)
ESTABLISHMENT OF AN EFFICIENT MEDIUM FOR ANTHER CULTURE OF RICE THROUGH COMPARATIVE EXPERIMENTS ON THE NITROGEN SOURCESScience of Sintering, 18
T. Komari (1990)
Transformation of cultured cells of Chenopodium quinoa by binary vectors that carry a fragment of DNA from the virulence region of pTiBo542Plant Cell Reports, 9
V. Masteller, D. Holden (1970)
The growth of and organ formation from callus tissue of sorghum.Plant physiology, 45 3
David Johnson, J. Gautsch, J. Sportsman, J. Elder (1984)
Improved technique utilizing nonfat dry milk for analysis of proteins and nucleic acids transferred to nitrocelluloseGene Analysis Techniques, 1
M. Cheng, J. Fry, Shengzhi Pang, Hua-Ping Zhou, C. Hironaka, D. Duncan, T. Conner, Y. Wan (1997)
Genetic Transformation of Wheat Mediated by Agrobacterium tumefaciens, 115
Y. Ishida, H. Saito, S. Ohta, Y. Hiei, T. Komari, T. Kumashiro (1996)
High efficiency transformation of maize (Zea mays L.) mediated by Agrobacterium tumefaciensNature Biotechnology, 14
H. Hadley (1953)
Cytological Relationships Between Sorghum vulgare and S. halepense1Agronomy Journal, 45
M. Chan, Hsin-Hsiung Chang, S. Ho, W. Tong, Su-May Yu (1993)
Agrobacterium-mediated production of transgenic rice plants expressing a chimeric α-amylase promoter/β-glucuronidase genePlant Molecular Biology, 22
J. Botterman, V. Gosselé, C. Thoen, M. Lauwereys (1991)
Characterization of phosphinothricin acetyltransferase and C-terminal enzymatically active fusion proteins.Gene, 102 1
G. Vancanneyt, R. Schmidt, A. O’Connor-Sánchez, L. Willmitzer, M. Rocha-Sosa (2004)
Construction of an intron-containing marker gene: Splicing of the intron in transgenic plants and its use in monitoring early events in Agrobacterium-mediated plant transformationMolecular and General Genetics MGG, 220
Jhy-Jhu Lin, N. Assad-Garcia, J. Kuo (1995)
Plant hormone effect of antibiotics on the transformation efficiency of plant tissues by Agrobacterium tumefaciens cellsPlant Science, 109
I. Godwin, R. Chikwamba (1994)
Transgenic Grain Sorghum (Sorghum bicolor) Plants via Agrobacterium
D.C. Montgomery (1997)
Design and Analysis of Experiments
D. Bidney, C. Scelonge, J. Martich, M. Burrus, Lynn Sims, G. Huffman (2004)
Microprojectile bombardment of plant tissues increases transformation frequency by Agrobacterium tumefaciensPlant Molecular Biology, 18
Agrobacterium tumefaciens was used to genetically transform sorghum. Immature embryos of a public (P898012) and a commercial line (PHI391) of sorghum were used as the target explants. The Agrobacterium strain used was LBA4404 carrying a `Super-binary' vector with a bar gene as a selectable marker for herbicide resistance in the plant cells. A series of parameter tests was used to establish a baseline for conditions to be used in stable transformation experiments. A number of different transformation conditions were tested and a total of 131 stably transformed events were produced from 6175 embryos in these two sorghum lines. Statistical analysis showed that the source of the embryos had a very significant impact on transformation efficiency, with field-grown embryos producing a higher transformation frequency than greenhouse-grown embryos. Southern blot analysis of DNA from leaf tissues of T0 plants confirmed the integration of the T-DNA into the sorghum genome. Mendelian segregation in the T1 generation was confirmed by herbicide resistance screening. This is the first report of successful use of Agrobacterium for production of stably transformed sorghum plants. The Agrobacterium method we used yields a higher frequency of stable transformation that other methods reported previously.
Plant Molecular Biology – Springer Journals
Published: Oct 16, 2004
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