1062-3604/03/3401- $25.00 © 2003
Russian Journal of Developmental Biology, Vol. 34, No. 1, 2003, pp. 19–21. Translated from Ontogenez, Vol. 34, No. 1, 2003, pp. 24–27.
Original Russian Text Copyright © 2003 by Toivonen, Sidorov, Nefedova, Yurovitskii.
The data on lipid and fatty acid composition of the
salmon lenses are essential for comprehensive analysis
of the ontogenetic features of metabolism, as well as for
studies of biological mechanisms of various patholo-
gies in the course of development. Here, we present the
data on changes in the fatty acid composition of the
lenses of one- and two-year salmons suggesting the
substitution of highly unsaturated acyls by the little
unsaturated ones. This indicates to an age-related
increased rigidity of membrane packaging of the lens
MATERIALS AND METHODS
Studies were carried on lenses of salmon underyear-
lings and yearlings grown at the Vygskii hatchery
For analysis of lipids, the materials were homoge-
nized and ﬁxed by a chloroform–methanol mixture.
Lipids were extracted as described by Folch
(1957). Total lipids were divided into fractions using
unidimensional thin-layer chromatography. Total phos-
pholipids, triacylglycerols, and cholesterol esters were
quantitated by the hydroxamate method (Sidorov
1972). Cholesterol was assayed according to the reac-
tion with a dye (Engelbrecht
, 1974) individual
phospholipids were assayed according to phosphorus
1966). The fatty acid composition of lip-
ids was determined using gas-liquid chromatography
(Tsyganov, 1971). Nonesteriﬁed fatty acids were
assayed as described by Prokhorov
converting them into copper salts with subsequent for-
mation of stained complexes in the presence of a
reagent to copper.
The products of lipid peroxidation were registered
using the color reaction of 2-thiobarbituric acid with
malone dialdehyde (Vladimirov and Archakov, 1972).
The protein concentration was determined by the
Bradford (1976) method based on binding of Cou-
massie blue G-250 to proteins.
The lens homogenates were gel-ﬁltered on a column
with Ultragel AcA-34 on an LKB chromatographer
(Pharmacia, Sweden). The column (2.5
95 cm) was
calibrated using a mixture of proteins with known
molecular mass (trypsin, serum albumin, and immuno-
globulin) at the elution rate 20 ml/h.
The experimental results were statistically pro-
cessed using the standard methods (Matyushichev,
1990) and the samples were also compared using non-
parameteric Wilcoxon-Mann-Whitney test (Gubler and
The differences between the indices given in tables
in the compared variants are signiﬁcant with reference
to the control at
RESULTS AND DISCUSSION
Content of lipids and age-related dynamics of indi-
vidual classes of lipids
A study of the lipid composi-
tion of lenses has revealed a number of age-related spe-
Age-Related Features of Cataractogenesis in the Salmon Fry.
1. Lipid Composition of Lens during Normal Development
L. V. Toivonen*, V. S. Sidorov**
, Z. A. Nefedova**, and Yu. G. Yurovitskii***
Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences,
pr. M. Toreza 44, St. Petersburg, 194223 Russia
**Institute of Biology, Karel Scientiﬁc Center of the Russian Academy of Sciences,
ul. Pushkinskaya 11, Petrozavodsk, 185610 Russia
***Kol’tsov Institute of Developmental Biology, Russian Academy of Sciences, ul. Vavilova 26, Moscow, 119991 Russia
Received October 19, 2000; in ﬁnal form, May 24, 2001
—This papers opens a series of publications on the mechanisms of cataractogenesis in the salmon fry.
Biochemical features of normal lens development and cataractogenesis in fry of different age and age-related
dynamics of the liver lipid composition during upon cataractogenesis will be dealt with, since lipids are most
intensely synthesized in the liver, from where they are transported in the lens. Here, we describe the dynamics
of lens lipid composition in the salmon fry, including the total lipid content and dynamics of individual classes.
The data are analyzed on the fatty acid spectrum of the salmon fry lens, as compared to the human lens.
: lens, lipid metabolism, salmon fry.