Aerobic training improves platelet function in type 2 diabetic patients:
role of microRNA-130a and GPIIb
· Mehdi Kargarfard
· Mahmood Naderi
Received: 20 April 2018 / Accepted: 23 May 2018
© Springer-Verlag Italia S.r.l., part of Springer Nature 2018
Aims MicroRNAs (miRs) that are mediators of gene expression have been implicated in type 2 diabetes mellitus (T2DM).
Platelet hyper-reactivity is one of the most important disorders in T2DM patients. In this study, we explored the eﬀects of
aerobic training (AT) on platelet aggregation and Glycoprotein IIb (GPIIb) receptor and miR-130a expression.
Methods In a quasi-experimental controlled trial, 24 sedentary, eligible female participants with T2DM were selected (age
61.92 ± 3.63) and divided into AT and control (CON) groups based on their peak oxygen consumption (VO
). AT proto-
col was performed three times per week in non-consecutive days on a treadmill with mean intensity (60–75% VO
) for 8
weeks, while the control group refrained from any type of exercise training. Two blood samples were taken before and after
this period. Real-time PCR was used to determine the expression of platelet GPIIb and miR-130a. Moreover, platelet indices
(PLT, MPV, PDW, and PCT), collagen-induced platelet aggregation and glycemic variables were measured.
Results Analyses of data showed that anthropometric variables, VO
and glycemic control improved signiﬁcantly
(P < 0.01) after AT. Furthermore, MPV, PDW (P < 0.01), and platelet aggregation (P < 0.001) decreased signiﬁcantly fol-
lowing AT compared with control group. Platelet GPIIb expression down-regulated signiﬁcantly (P < 0.05) in AT group but
up-regulation of miR-130a expression was not signiﬁcant between two groups (P > 0.05).
Conclusions Platelet hyper-reactivity in T2DM females might be decreased not only by glycemic control and amelioration of
anthropometric and platelet indices, but also the down-regulation of GPIIb following AT. However, more research is needed
to determine the eﬀects of exercise training on platelet miR-130a.
Keywords Platelet indices · GPIIb/IIIa · MicroRNA-130a · Exercise training · Diabetes mellitus
In the twenty-ﬁrst century, non-communicable diseases such
as diabetes and cardiovascular disorders are the main pub-
lic health menace. Type two diabetes mellitus (T2DM) is a
metabolic disease and characterized by hyperglycemia due
to insulin resistance (IR) and unnatural insulin secretion [1,
2]. T2DM has a rapid spread rate and is expected to aﬀect
more than 550 million people by 2030 . These patients
have a higher risk of cardiovascular disease (CVD), so that
about 70% of morbidity and mortality in T2DM were related
to CVD [3, 4]. One of the important CVD complications
in T2DM is located in a prothrombotic state that usually
attributes to platelet dysfunction [2, 4].
Platelets are anucleate cells and have a critical role in
thrombosis and hemostasis . In the normal condition,
circulating platelets are inactive. Following vascular dam-
age, platelet activation begins by binding agonists to their
receptors and starting various controlled signaling pathways
. GPIIb/IIIa complex (Integrin ɑ
) is an abundant
(50,000–60,000 per platelet) receptor that is found on the
surface of platelets [6, 7] and plays an important role in
platelet function and aggregation . This complex com-
poses of two discrete subunits GPIIb (ɑ
) and GPIIIa (β
. The previous investigations suggest that one of the
most common platelet disorders in T2DM is the increased
Managed by Massimo Porta.
* Mehdi Kargarfard
Department of Exercise Physiology, Faculty of Sport
Sciences, University of Isfahan, Isfahan, Iran
Cell-Based Therapies Research Center, Digestive Disease
Research Institute, Tehran University of Medical Sciences,