Advances in synthetic biology of oleaginous yeast Yarrowia lipolytica for producing non-native chemicals

Advances in synthetic biology of oleaginous yeast Yarrowia lipolytica for producing non-native... Oleaginous yeast Yarrowia lipolytica is an important industrial host for the production of enzymes, oils, fragrances, surfac- tants, cosmetics, and pharmaceuticals. More recently, improved synthetic biology tools have allowed more extensive engineering of this yeast species, which lead to the production of non-native metabolites. In this review, we summarize the recent advances of genome editing tools for Y. lipolytica, including the application of CRISPR/Cas9 system and discuss case studies, where Y. lipolytica was engineered to produce various non-native chemicals: short-chain fatty alcohols and alkanes as biofuels, polyunsaturated fatty acids for nutritional and pharmaceutical applications, polyhydroxyalkanoates and dicarboxylic acids as precursors for biodegradable plastics, carotenoid-type pigments for food and feed, and campesterol as a precursor for steroid drugs. . . . Keywords Yarrowia lipolytica Synthetic biology CRISPR/Cas9 Bio-based chemicals Introduction Belgium), erythritol (Baolingbao Biology Co., China), fodder yeast, and probiotics (Skotan SA, Poland). British Petroleum Yarrowia lipolytica is rapidly gaining popularity as an indus- (UK) produced single-cell proteins from this yeast for live- trial host for production of recombinant proteins and other stock feeding (Toprina G) until 1978 (Groenewald et al. chemicals (Darvishi et al. 2017;Madzak 2015). This oleagi- 2014;DarvishiHarzevili 2014). Furthermore, genetically nous yeast species has an exceptional ability http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Microbiology and Biotechnology Springer Journals

Advances in synthetic biology of oleaginous yeast Yarrowia lipolytica for producing non-native chemicals

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Publisher
Springer Berlin Heidelberg
Copyright
Copyright © 2018 by Springer-Verlag GmbH Germany, part of Springer Nature
Subject
Life Sciences; Microbiology; Microbial Genetics and Genomics; Biotechnology
ISSN
0175-7598
eISSN
1432-0614
D.O.I.
10.1007/s00253-018-9099-x
Publisher site
See Article on Publisher Site

Abstract

Oleaginous yeast Yarrowia lipolytica is an important industrial host for the production of enzymes, oils, fragrances, surfac- tants, cosmetics, and pharmaceuticals. More recently, improved synthetic biology tools have allowed more extensive engineering of this yeast species, which lead to the production of non-native metabolites. In this review, we summarize the recent advances of genome editing tools for Y. lipolytica, including the application of CRISPR/Cas9 system and discuss case studies, where Y. lipolytica was engineered to produce various non-native chemicals: short-chain fatty alcohols and alkanes as biofuels, polyunsaturated fatty acids for nutritional and pharmaceutical applications, polyhydroxyalkanoates and dicarboxylic acids as precursors for biodegradable plastics, carotenoid-type pigments for food and feed, and campesterol as a precursor for steroid drugs. . . . Keywords Yarrowia lipolytica Synthetic biology CRISPR/Cas9 Bio-based chemicals Introduction Belgium), erythritol (Baolingbao Biology Co., China), fodder yeast, and probiotics (Skotan SA, Poland). British Petroleum Yarrowia lipolytica is rapidly gaining popularity as an indus- (UK) produced single-cell proteins from this yeast for live- trial host for production of recombinant proteins and other stock feeding (Toprina G) until 1978 (Groenewald et al. chemicals (Darvishi et al. 2017;Madzak 2015). This oleagi- 2014;DarvishiHarzevili 2014). Furthermore, genetically nous yeast species has an exceptional ability

Journal

Applied Microbiology and BiotechnologySpringer Journals

Published: May 28, 2018

References

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