Advances in serological, imaging techniques and molecular diagnosis of Toxoplasma gondii infection

Advances in serological, imaging techniques and molecular diagnosis of Toxoplasma gondii infection Background Toxoplasmosis is worldwide distributed zoonotic infection disease with medical importance in immunocom- promised patients, pregnant women and congenitally infected newborns. Having basic information on the traditional and new developed methods is essential for general physicians and infectious disease specialists for choosing a suitable diagnostic approach for rapid and accurate diagnosis of the disease and, consequently, timely and effective treatment. Methods We conducted English literature searches in PubMed from 1989 to 2016 using relevant keywords and summarized the recent advances in diagnosis of toxoplasmosis. Results Enzyme-linked immunosorbent assay (ELISA) was most used method in past century. Recently advanced ELISA- based methods including chemiluminescence assays (CLIA), enzyme-linked fluorescence assay (ELFA), immunochroma - tographic test (ICT), serum IgG avidity test and immunosorbent agglutination assays (ISAGA) have shown high sensitivity and specificity. Recent studies using recombinant or chimeric antigens and multiepitope peptides method demonstrated very promising results to development of new strategies capable of discriminating recently acquired infections from chronic infec- tion. Real-time PCR and loop-mediated isothermal amplification (LAMP) are two recently developed PCR-based methods with high sensitivity and specificity and could be useful to early diagnosis of infection. Computed tomography, magnetic resonance imaging, nuclear imaging and ultrasonography could be useful, although http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Infection Springer Journals

Advances in serological, imaging techniques and molecular diagnosis of Toxoplasma gondii infection

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Publisher
Springer Berlin Heidelberg
Copyright
Copyright © 2018 by Springer-Verlag GmbH Germany, part of Springer Nature
Subject
Medicine & Public Health; Infectious Diseases; General Practice / Family Medicine; Internal Medicine
ISSN
0300-8126
eISSN
1439-0973
D.O.I.
10.1007/s15010-017-1111-3
Publisher site
See Article on Publisher Site

Abstract

Background Toxoplasmosis is worldwide distributed zoonotic infection disease with medical importance in immunocom- promised patients, pregnant women and congenitally infected newborns. Having basic information on the traditional and new developed methods is essential for general physicians and infectious disease specialists for choosing a suitable diagnostic approach for rapid and accurate diagnosis of the disease and, consequently, timely and effective treatment. Methods We conducted English literature searches in PubMed from 1989 to 2016 using relevant keywords and summarized the recent advances in diagnosis of toxoplasmosis. Results Enzyme-linked immunosorbent assay (ELISA) was most used method in past century. Recently advanced ELISA- based methods including chemiluminescence assays (CLIA), enzyme-linked fluorescence assay (ELFA), immunochroma - tographic test (ICT), serum IgG avidity test and immunosorbent agglutination assays (ISAGA) have shown high sensitivity and specificity. Recent studies using recombinant or chimeric antigens and multiepitope peptides method demonstrated very promising results to development of new strategies capable of discriminating recently acquired infections from chronic infec- tion. Real-time PCR and loop-mediated isothermal amplification (LAMP) are two recently developed PCR-based methods with high sensitivity and specificity and could be useful to early diagnosis of infection. Computed tomography, magnetic resonance imaging, nuclear imaging and ultrasonography could be useful, although

Journal

InfectionSpringer Journals

Published: Jan 12, 2018

References

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