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V Cerundolo, JD Silk, SH Masri, M Salio (2009)
Harnessing invariant NKT cells in vaccination strategiesNat Rev Immunol, 9
M Naderi, A Saeedi, A Moradi, M Kleshadi, MR Zolfaghari, A Gorji, A Ghaemi (2013)
Interleukin-12 as a genetic adjuvant enhances hepatitis C virus NS3 DNA vaccine immunogenicityVirol Sin, 28
FB Scorza, V Tsvetnitsky, JJ Donnelly (2016)
Universal influenza vaccines: shifting to better vaccinesVaccine, 34
K Xu, Z-Y Ling, L Sun, Y Xu, C Bian, Y He, W Lu, Z Chen, B Sun (2011)
Broad humoral and cellular immunity elicited by a bivalent DNA vaccine encoding HA and NP genes from an H5N1 virusViral Immunol, 24
K Bandyopadhyay, I Marrero, V Kumar (2016)
NKT cell subsets as key participants in liver physiology and pathologyCell Mol Immunol, 13
WA Fischer, MG II, S Bhagwanjee, J Sevransky (2014)
Global burden of influenza: contributions from resource limited and low-income settingsGlob Heart, 9
LJMH Reed (1938)
A simple method of estimating fifty percent endpointsAm J Hyg, 27
Z Stanekova, E Vareckova (2010)
Conserved epitopes of influenza A virus inducing protective immunity and their prospects for universal vaccine developmentVirol J, 7
A Tahamtan, A Ghaemi, A Gorji, HR Kalhor, A Sajadian, A Tabarraei, A Moradi, F Atyabi, M Kelishadi (2014)
Antitumor effect of therapeutic HPV DNA vaccines with chitosan-based nanodelivery systemsJ Biomed Sci, 21
M-C Kim, J-M Song, O Eunju, Y-M Kwon, Y-J Lee, RW Compans, S-M Kang (2013)
Virus-like particles containing multiple M2 extracellular domains confer improved cross-protection against various subtypes of influenza virusMol Ther, 21
BA Sullivan, M Kronenberg (2005)
Activation or anergy: NKT cells are stunned by α-galactosylceramideJ Clin Investig, 115
GZ Tau, SN Cowan, J Weisburg, NS Braunstein, PB Rothman (2001)
Regulation of IFN-γ signaling is essential for the cytotoxic activity of CD8+T cellsJ Immunol, 167
C Guillonneau, JD Mintern, F-X Hubert, AC Hurt, GS Besra, S Porcelli, IG Barr, PC Doherty, DI Godfrey, SJ Turner (2009)
Combined NKT cell activation and influenza virus vaccination boosts memory CTL generation and protective immunityProc Natl Acad Sci, 106
C Carnaud, D Lee, O Donnars, S-H Park, A Beavis, Y Koezuka, A Bendelac (1999)
Cutting edge: cross-talk between cells of the innate immune system: NKT cells rapidly activate NK cellsJ Immunol, 163
L Deng, KJ Cho, W Fiers, X Saelens (2015)
M2e-based universal influenza A vaccinesVaccines, 3
T Mohan, P Verma, DN Rao (2013)
Novel adjuvants and delivery vehicles for vaccines development: a road aheadIndian J Med Res, 138
JS Im, P Arora, G Bricard, A Molano, MM Venkataswamy, I Baine, ES Jerud, MF Goldberg, A Baena, O Karl (2009)
Kinetics and cellular site of glycolipid loading control the outcome of natural killer T cell activationImmunity, 30
H Chen, M Angel, W Li, C Finch, AS Gonzalez, T Sutton, J Santos, DR Perez (2014)
All-in-one bacmids: an efficient reverse genetics strategy for influenza A virus vaccinesJ Virol, 88
SA Kopecky-Bromberg, KA Fraser, N Pica, E Carnero, TM Moran, RW Franck, M Tsuji, P Palese (2009)
Alpha-C-galactosylceramide as an adjuvant for a live attenuated influenza virus vaccineVaccine, 27
JW Huleatt, V Nakaar, P Desai, Y Huang, D Hewitt, A Jacobs, J Tang, W McDonald, L Song, RK Evans, S Umlauf, L Tussey, TJ Powell (2008)
Potent immunogenicity and efficacy of a universal influenza vaccine candidate comprising a recombinant fusion protein linking influenza M2e to the TLR5 ligand flagellinVaccine, 26
H Kamijuku, Y Nagata, X Jiang, T Ichinohe, T Tashiro, K Mori, M Taniguchi, K Hase, H Ohno, T Shimaoka (2008)
Mechanism of NKT cell activation by intranasal coadministration of α-galactosylceramide, which can induce cross-protection against influenza virusesMucosal Immunol, 1
LE Brown, A Kelso (2009)
Prospects for an influenza vaccine that induces cross-protective cytotoxic T lymphocytesImmunol Cell Biol, 87
Y Huang, A Chen, X Li, Z Chen, W Zhang, Y Song, D Gurner, D Gardiner, S Basu, DD Ho (2008)
Enhancement of HIV DNA vaccine immunogenicity by the NKT cell ligand, α-galactosylceramideVaccine, 26
A Iwasaki, PS Pillai (2014)
Innate immunity to influenza virus infectionNat Rev Immunol, 14
MA Exley (2012)
Editorial: NKT get the ‘flu: NKT cells as (mostly) good guys in influenza; monocytic cells as double agentsJ Leukoc Biol, 91
MA Kutzler, DB Weiner (2008)
DNA vaccines: ready for prime time?Nat Rev Genet, 9
EA Hemann, SM Kang, KL Legge (2013)
Protective CD8 T cell-mediated immunity against influenza A virus infection following influenza virus-like particle vaccinationJ Immunol (Baltimore, Md: 1950), 191
A Chackerian, J Alt, V Perera, SM Behar (2002)
Activation of NKT cells protects mice from tuberculosisInfect Immun, 70
L Kaer, VV Parekh, L Wu (2011)
Invariant NK T cells: potential for immunotherapeutic targeting with glycolipid antigensImmunotherapy, 3
J Denis, E Acosta-Ramirez, Y Zhao, ME Hamelin, I Koukavica, M Baz, Y Abed, C Savard, C Pare, C Lopez Macias, G Boivin, D Leclerc (2008)
Development of a universal influenza A vaccine based on the M2e peptide fused to the papaya mosaic virus (PapMV) vaccine platformVaccine, 26
FC Robertson, JA Berzofsky, M Terabe (2014)
NKT cell networks in the regulation of tumor immunityFront Immunol, 5
J-T Hung, Y-C Tsai, W-D Lin, J-T Jan, K-H Lin, J-R Huang, J-Y Cheng, M-W Chen, C-H Wong, LY Alice (2014)
Potent adjuvant effects of novel NKT stimulatory glycolipids on hemagglutinin based DNA vaccine for H5N1 influenza virusAntivir Res, 107
F Gableh, M Saeidi, S Hemati, K Hamdi, H Soleimanjahi, A Gorji, A Ghaemi (2016)
Combination of the toll like receptor agonist and alpha-Galactosylceramide as an efficient adjuvant for cancer vaccineJ Biomed Sci, 23
A Saeedi, A Ghaemi, A Tabarraei, A Moradi, A Gorji, S Semnani, H Soleimanjahi, AH Adli, SY Hosseini, MA Vakili (2014)
Enhanced cell immune responses to hepatitis C virus core by novel heterologous DNA prime/lambda nanoparticles boost in miceVirus Genes, 49
A-MC Andersson, KO Håkansson, BAH Jensen, D Christensen, P Andersen, AR Thomsen, JP Christensen (2012)
Increased immunogenicity and protective efficacy of influenza M2e fused to a tetramerizing proteinPloS One, 7
A Holvast, A Haan, S Assen, CA Stegeman, MG Huitema, A Huckriede, CA Benne, J Westra, A Palache, J Wilschut (2010)
Cell-mediated immune responses to influenza vaccination in Wegener’s granulomatosisAnn Rheum Dis, 69
C Santo, M Salio, SH Masri, LY Lee, T Dong, AO Speak, S Porubsky, S Booth, N Veerapen, GS Besra, HJ Grone, FM Platt, M Zambon, V Cerundolo (2008)
Invariant NKT cells reduce the immunosuppressive activity of influenza A virus-induced myeloid-derived suppressor cells in mice and humansJ Clin Investig, 118
L Shang, Q Liu, W Liu, J Men, S Gao, L Jiang, Z Wang, Y Zhai, H Jin, H Lian, C Chen, Z Xia, Z Yuan, XQ Zhu (2009)
Protection in mice immunized with a heterologous prime-boost regime using DNA and recombinant pseudorabies expressing TgSAG1 against Toxoplasma gondii challengeVaccine, 27
S-Y Ko, H-J Ko, W-S Chang, S-H Park, M-N Kweon, C-Y Kang (2005)
α-Galactosylceramide can act as a nasal vaccine adjuvant inducing protective immune responses against viral infection and tumorJ Immunol, 175
V Dwivedi, C Manickam, S Dhakal, B Binjawadagi, K Ouyang, J Hiremath, M Khatri, JG Hague, CW Lee, GJ Renukaradhya (2016)
Adjuvant effects of invariant NKT cell ligand potentiates the innate and adaptive immunity to an inactivated H1N1 swine influenza virus vaccine in pigsVet Microbiol, 186
A Sajadian, A Tabarraei, H Soleimanjahi, F Fotouhi, A Gorji, A Ghaemi (2014)
Comparing the effect of Toll-like receptor agonist adjuvants on the efficiency of a DNA vaccineArch Virol, 159
A Ghaemi, H Soleimanjahi, T Bamdad, S Soudi, E Arefeian, SM Hashemi, M Ebtekar (2007)
Induction of humoral and cellular immunity against latent HSV-1 infections by DNA immunization in BALB/c miceComp Immunol Microbiol Infect Dis, 30
FS Dawood, AD Iuliano, C Reed, MI Meltzer, DK Shay, P-Y Cheng, D Bandaranayake, RF Breiman, WA Brooks, P Buchy (2012)
Estimated global mortality associated with the first 12 months of 2009 pandemic influenza A H1N1 virus circulation: a modelling studyLancet Infect Dis, 12
Q Chen, KL Mosovsky, AC Ross (2011)
Retinoic acid and α-galactosylceramide differentially regulate B cell activation in vitro and augment antibody production in vivoClin Vaccine Immunol, 18
DNA vaccines can induce both humoral and cellular immune responses in animals. However, DNA vaccines suffer from limited vaccine potency due to low immunogenicity. Therefore, different strategies are required for significant improvement of DNA vaccine efficacy such as inclusion of strong adjuvants. The aim of the present study was to investigate the effects of using α-Galactosylceramide (α-GalCer) as an adjuvant to enhance the immune responses induced by a DNA vaccine, encoding influenza A virus matrix protein 2 (M2), against influenza A challenge. BALB/c mice were immunized three times by intramuscular inoculations of DNA vaccine encoding M2 alone or in combination with α-GalCer adjuvant. The adjuvant effect was evaluated by measuring the serum antibody titers, using ELISA, lymphocyte proliferation, using MTT assay as well as Th1 (IFN-γ and IL-12) and Th2 (IL-4) cytokines. The results showed that co-administration of α-GalCer with the vaccine exert protective effects by influencing the magnitude and quality of humoral responses. Adjuvanted DNA-vaccinated mice revealed a higher IgG titer and IgG2a/IgG1 ratio than mice vaccinated with DNA alone. Furthermore, analysis of M2-specific responses revealed that the DNA vaccine triggered predominately IgG1 and IL-4 responses indicating a Th2 bias. The data also showed that α-GalCer is a potent adjuvant for activation of cellular immune responses to DNA vaccine. This was supported by a higher IgG2a/IgG1 ratio, significantly increased IFN-γ and IL-4 production and CD4+ proliferation, compared with mice receiving the DNA vaccine alone, suggesting a mixed Th1/Th2-type cellular immune response with a Th1 bias. The findings of this study indicate that α-GalCer has the potential to be used as a potent adjuvant for a DNA vaccine encoding M2, since it enhances humoral and cellular immune response and improves immune protection against influenza challenge in mice.
Archives of Virology – Springer Journals
Published: Jan 24, 2017
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