Activity of Membrane-Associated Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase during Development of the Inner Membrane System in Potato Chloroplasts

Activity of Membrane-Associated Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase during... Membranes of etioplasts and chloroplasts from potato plants (Solanum tuberosumL., cv. Zhu-kovskii) were fractionated by centrifugation in a sucrose gradient, and the fractions of primary membranes, fretlike structures, and frets and granal thylakoids were isolated. The carboxylase and oxygenase activities of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) were measured in these fractions. The membrane-bound Rubisco (mRubisco) was reconstructed in the artificial system from the primary membranes and the soluble form of Rubisco (sRubisco), both isolated from the chloroplasts. The higher carboxylase and lower oxygenase activities were found to be characteristic of this reconstructed mRubisco as compared to sRubisco. The degree of sRubisco association with primary membranes depended on the Mg2+concentration. The data suggested that the association of sRubisco with primary membranes occurred at the early stages of membrane formation. The biological role of mRubisco is assumed to consist in controlling the ratio between photosynthesis and photorespiration at the membrane level in plants of various physiological states. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Activity of Membrane-Associated Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase during Development of the Inner Membrane System in Potato Chloroplasts

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Publisher
Springer Journals
Copyright
Copyright © 2001 by MAIK “Nauka/Interperiodica”
Subject
Life Sciences; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1023/A:1009035630151
Publisher site
See Article on Publisher Site

Abstract

Membranes of etioplasts and chloroplasts from potato plants (Solanum tuberosumL., cv. Zhu-kovskii) were fractionated by centrifugation in a sucrose gradient, and the fractions of primary membranes, fretlike structures, and frets and granal thylakoids were isolated. The carboxylase and oxygenase activities of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) were measured in these fractions. The membrane-bound Rubisco (mRubisco) was reconstructed in the artificial system from the primary membranes and the soluble form of Rubisco (sRubisco), both isolated from the chloroplasts. The higher carboxylase and lower oxygenase activities were found to be characteristic of this reconstructed mRubisco as compared to sRubisco. The degree of sRubisco association with primary membranes depended on the Mg2+concentration. The data suggested that the association of sRubisco with primary membranes occurred at the early stages of membrane formation. The biological role of mRubisco is assumed to consist in controlling the ratio between photosynthesis and photorespiration at the membrane level in plants of various physiological states.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Oct 10, 2004

References

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