In species of the Annonaceae family, particularly Annona diversifolia Safford, benzylisoquinoline alkaloids (BIA) are secondary metabolites that appear to contribute to the phytopathogen defense mechanisms of plants. Polyphenol oxidase (PPO, EC 184.108.40.206), amine oxidase (AO, EC 220.127.116.11), tyrosine decarboxylase (TYDC, EC 18.104.22.168), and norcoclaurine synthase (NCS, EC 22.214.171.124) catalyze the initial steps in BIA biosynthesis. This study reports the activity of these enzymes in different plant organs at four stages of the early development of A. diversifolia seedlings: seeds imbibed for 5 days, seeds after 3 days of germination, seedlings with leaf primordia, and seedlings with two true leaves. Evaluations were performed according to specific protocols for each of the enzymes. All four enzymes were active in the developing embryos during imbibition and germination, but no activity was detected in the endosperm. In seedlings with leaf primordia and seedlings with two true leaves (25 and 30 days after the start of imbibition, respectively), the activities of three enzymes (TYDC, PPO, and AO) were observed in all of the tissues, while NCS activity was only observed in the stems and roots. The activities of these enzymes in embryos provides evidence that alkaloid biosynthesis at early developmental stages is related to embryo growth and development. This study is the first report that has described some aspects of alkaloid biosynthesis in Annonaceae.
Russian Journal of Plant Physiology – Springer Journals
Published: Oct 13, 2013
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera