Activation of the expression of the microcin c51 operon upon glucose starvation of cells at the exponential growth phase

Activation of the expression of the microcin c51 operon upon glucose starvation of cells at the... It was earlier shown that expression of the microcin C51 operon in Escherichia coli cells is activated upon decelerated growth of cells during their transition to the stationary growth phase and depends on the σS subunit of RNA polymerase. Using a single-copy construct containing the cloned promoter region of the microcin C51 operon and a promoterless lac operon ( Pmcc-lac ), it was shown that the promoter of the microcin operon was also induced by stress caused by the transition of cells at the exponential growth phase into the medium without glucose as a sole carbon source. Activation of Pmcc-lac expression upon severe glucose starvation occurred in rpoS + and rpoS – strains. In cells carrying the rpoD800 mutation that renders the σ70 subunit of RNA polymerase temperature-sensitive, an activation of Pmcc-lac expression was observed at nonpermissive temperature, in contrast to its complete inhibition in E. coli cells at the phase of delayed growth. Other stressors —nitrogen starvation, high temperatures, osmotic shock, tetracycline and chloramphenicol—did not activate Pmcc-lac expression in cells at the exponential growth phase. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Genetics Springer Journals

Activation of the expression of the microcin c51 operon upon glucose starvation of cells at the exponential growth phase

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Publisher
Nauka/Interperiodica
Copyright
Copyright © 2005 by MAIK “Nauka/Interperiodica”
Subject
Biomedicine; Human Genetics; Microbial Genetics and Genomics; Animal Genetics and Genomics
ISSN
1022-7954
eISSN
1608-3369
D.O.I.
10.1007/PL00022108
Publisher site
See Article on Publisher Site

Abstract

It was earlier shown that expression of the microcin C51 operon in Escherichia coli cells is activated upon decelerated growth of cells during their transition to the stationary growth phase and depends on the σS subunit of RNA polymerase. Using a single-copy construct containing the cloned promoter region of the microcin C51 operon and a promoterless lac operon ( Pmcc-lac ), it was shown that the promoter of the microcin operon was also induced by stress caused by the transition of cells at the exponential growth phase into the medium without glucose as a sole carbon source. Activation of Pmcc-lac expression upon severe glucose starvation occurred in rpoS + and rpoS – strains. In cells carrying the rpoD800 mutation that renders the σ70 subunit of RNA polymerase temperature-sensitive, an activation of Pmcc-lac expression was observed at nonpermissive temperature, in contrast to its complete inhibition in E. coli cells at the phase of delayed growth. Other stressors —nitrogen starvation, high temperatures, osmotic shock, tetracycline and chloramphenicol—did not activate Pmcc-lac expression in cells at the exponential growth phase.

Journal

Russian Journal of GeneticsSpringer Journals

Published: Feb 24, 2005

References

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