Activation of genes encoding mitochondrial proteins involved in alternative and uncoupled respiration of tomato plants treated with low temperature and reactive oxygen species

Activation of genes encoding mitochondrial proteins involved in alternative and uncoupled... Using Real-time PCR, the regulation of gene expression for proteins involved in alternative and uncoupled respiration in tomato (Lycopersicon esculentum Mill.) seedlings and cell culture was studied. The temperature of 4°C activated transcription of these genes. The effect of low temperature on the activities of a number of enzymes involved in oxidative metabolism of seedlings and cultivated cells was detected by spec-trophotometric methods. When oxidative stress in tomato cell culture was created by treatments with hydrogen peroxide and antimycin A, it was established that the mRNA levels of the alternative oxidase, the uncoupling protein, and the ATP/ADP antiporter were controlled by ROS, and this may be the mechanism of “free” oxidation induction during cold stress adaptation. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Activation of genes encoding mitochondrial proteins involved in alternative and uncoupled respiration of tomato plants treated with low temperature and reactive oxygen species

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Publisher
SP MAIK Nauka/Interperiodica
Copyright
Copyright © 2011 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Plant Physiology; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443711040091
Publisher site
See Article on Publisher Site

Abstract

Using Real-time PCR, the regulation of gene expression for proteins involved in alternative and uncoupled respiration in tomato (Lycopersicon esculentum Mill.) seedlings and cell culture was studied. The temperature of 4°C activated transcription of these genes. The effect of low temperature on the activities of a number of enzymes involved in oxidative metabolism of seedlings and cultivated cells was detected by spec-trophotometric methods. When oxidative stress in tomato cell culture was created by treatments with hydrogen peroxide and antimycin A, it was established that the mRNA levels of the alternative oxidase, the uncoupling protein, and the ATP/ADP antiporter were controlled by ROS, and this may be the mechanism of “free” oxidation induction during cold stress adaptation.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Aug 21, 2011

References

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