ACOS5 is required for primexine formation and exine pattern formation during microsporogenesis in Arabidopsis

ACOS5 is required for primexine formation and exine pattern formation during microsporogenesis in... Pollen exine, mainly composed of sporopollenin, plays important roles during microspore development. It has been reported that Acyl-CoA Synthetase5 (ACOS5) is required for sporopollenin biosynthesis in Arabidopsis. Here we show that ACOS5 is essential for primexine formation during Arabidopsis microspore development. Through genetic screen, we identified a point mutation of ACOS5 allele, acos5-2, showing abnormal microspore development. Its microspores were degenerated and aborted after released from the tetrads. Transmission electron microscopy showed that primexine formation was reduced in acos5-2 mutant as compared to that of the wild-type. Consequently, sporopollenin was aggregated and randomly deposited on the microspores. In situ hybridization indicated that the key regulators of tapetum development, DYT1 and TDF1, are required for the expression of ACOS5 in tapetum. Furthermore, the GUS reporter showed that the 593-bp promoter sequence was sufficient for the expression of ACOS5 in the anther. Our data provide evidence that ACOS5 is required for primexine formation and sporopollenin deposition during microspore development. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Plant Biology Springer Journals

ACOS5 is required for primexine formation and exine pattern formation during microsporogenesis in Arabidopsis

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Publisher
Springer Berlin Heidelberg
Copyright
Copyright © 2017 by Korean Society of Plant Biologists and Springer-Verlag GmbH Germany
Subject
Life Sciences; Plant Sciences; Plant Breeding/Biotechnology; Plant Genetics and Genomics; Plant Systematics/Taxonomy/Biogeography; Plant Ecology
ISSN
1226-9239
eISSN
1867-0725
D.O.I.
10.1007/s12374-016-0523-4
Publisher site
See Article on Publisher Site

Abstract

Pollen exine, mainly composed of sporopollenin, plays important roles during microspore development. It has been reported that Acyl-CoA Synthetase5 (ACOS5) is required for sporopollenin biosynthesis in Arabidopsis. Here we show that ACOS5 is essential for primexine formation during Arabidopsis microspore development. Through genetic screen, we identified a point mutation of ACOS5 allele, acos5-2, showing abnormal microspore development. Its microspores were degenerated and aborted after released from the tetrads. Transmission electron microscopy showed that primexine formation was reduced in acos5-2 mutant as compared to that of the wild-type. Consequently, sporopollenin was aggregated and randomly deposited on the microspores. In situ hybridization indicated that the key regulators of tapetum development, DYT1 and TDF1, are required for the expression of ACOS5 in tapetum. Furthermore, the GUS reporter showed that the 593-bp promoter sequence was sufficient for the expression of ACOS5 in the anther. Our data provide evidence that ACOS5 is required for primexine formation and sporopollenin deposition during microspore development.

Journal

Journal of Plant BiologySpringer Journals

Published: Aug 8, 2017

References

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