Acid-Sensitive Outwardly Rectifying Anion Channels in Human Erythrocytes

Acid-Sensitive Outwardly Rectifying Anion Channels in Human Erythrocytes Acid-sensitive outwardly rectifying anion channels (ASOR) have been described in several mammalian cell types. The present whole-cell patch-clamp study elucidated whether those channels are expressed in erythrocytes. To this end whole-cell recordings were made in human erythrocytes from healthy donors treated with low pH and high osmotic pressure. When the pipette solution had a reduced Cl− concentration, treatment of the cells with Cl−-containing normal and hyperosmotic (addition of sucrose and polyethelene glycol 1000 [PEG-1000] to the Ringer) media with low pH significantly increased the conductance of the cells at positive voltages. Channel activity was highest in the PEG-1000 media (95 and 300 mM PEG-1000, pH 4.5 and 4.3, respectively) where the current–voltage curves demonstrated strong outward rectification and reversed at −40 mV. Substitution of the Cl−-containing medium with Cl−-free medium resulted in a decrease of the conductance at hyperpolarizing voltages, a shift in reversal potential (to 0 mV) and loss of outward rectification. The chloride currents were inhibited by chloride channels blockers DIDS and NPPB (IC50 for both was ~1 mM) but not with niflumic acid and amiloride. The observations reveal expression of ASOR in erythrocytes. The Journal of Membrane Biology Springer Journals

Acid-Sensitive Outwardly Rectifying Anion Channels in Human Erythrocytes

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Copyright © 2009 by Springer Science+Business Media, LLC
Life Sciences; Human Physiology ; Biochemistry, general
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