Accelerated root growth induced by nitrate deficit is related to apoplast acidification

Accelerated root growth induced by nitrate deficit is related to apoplast acidification We measured the rate of growth, osmotic pressure, hydraulic conductance, longitudinal and transverse extensibility of barley (Hordeum vulgare L.) roots in Knop solution with nitrate and at substitution of NO 3 − with Cl−. During the first three days after NO 3 − removal, root growth acceleration was related to the increase in their longitudinal extensibility. It was shown that root exposure to buffer with pH 4.5 and also activation of H+ pump with naphthyl acetate imitated changes in extensibility induced by NO 3 − deficit. Earlier, we have demonstrated medium acidification near root surface and calculated its expected level (pH 4.5). This permits a supposition that the cause for changes in extensibility and root growth acceleration at NO 3 − deficit was apoplast acidification, evidently related to the ceasing of NO 3 − symport with H+ and activation of the plasmalemmal H+ pump. ABA did not affect root extensibility at pH 4.5; however, at pH 6.0, it was similar to the action of diethylstilbestrol, an inhibitor of H+ pump, and opposite to the action of NO 3 − deficit. Thus, the absence of ABA effects on root growth, in spite of its accumulation at NO 3 − deficit, could be explained by apoplast acidification as well. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Accelerated root growth induced by nitrate deficit is related to apoplast acidification

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Publisher
SP MAIK Nauka/Interperiodica
Copyright
Copyright © 2010 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Plant Sciences ; Plant Physiology
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443710040059
Publisher site
See Article on Publisher Site

Abstract

We measured the rate of growth, osmotic pressure, hydraulic conductance, longitudinal and transverse extensibility of barley (Hordeum vulgare L.) roots in Knop solution with nitrate and at substitution of NO 3 − with Cl−. During the first three days after NO 3 − removal, root growth acceleration was related to the increase in their longitudinal extensibility. It was shown that root exposure to buffer with pH 4.5 and also activation of H+ pump with naphthyl acetate imitated changes in extensibility induced by NO 3 − deficit. Earlier, we have demonstrated medium acidification near root surface and calculated its expected level (pH 4.5). This permits a supposition that the cause for changes in extensibility and root growth acceleration at NO 3 − deficit was apoplast acidification, evidently related to the ceasing of NO 3 − symport with H+ and activation of the plasmalemmal H+ pump. ABA did not affect root extensibility at pH 4.5; however, at pH 6.0, it was similar to the action of diethylstilbestrol, an inhibitor of H+ pump, and opposite to the action of NO 3 − deficit. Thus, the absence of ABA effects on root growth, in spite of its accumulation at NO 3 − deficit, could be explained by apoplast acidification as well.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Jul 7, 2010

References

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