Russian Journal of Developmental Biology, Vol. 36, No. 3, 2005, pp. 189–198. Translated from Ontogenez, Vol. 36, No. 3, 2005, pp. 230–240. Original Russian Text Copyright © 2005. Abstracts of Papers at the Conference of Young Scientists, Kol’tsov Institute of Developmental Biology, Russian Academy of Sciences (Moscow, December 8, 2004) A Study of Tissue Specificity of Biological Activity of Regulatory Proteins A. V. Borisenko Group of Regulatory Proteins, Kol’tsov Institute of Developmental Biology, Russian Academy of Sciences, ul. Vavilova 26, Moscow, 119991 Russia Regulatory proteins were isolated from the liver, preliminary salting-out and fractions of the correspond- lung, kidney, and small intestine tissues of Wistar rats ing acidic proteins (pH < 3.0) were collected. The stud- using the scheme of isolation and puriﬁcation devel- ied fractions were tested on two organ cultures, mouse oped by Yamskova et al. This scheme included the liver and lung tissues in vitro. The extracts of liver and obtaining of tissue extract, salting-out in a saturated lung and the fractions of acidic proteins isolated from ammonium sulfate solution, isoelectrofocusing, and them were shown to express issue speciﬁcity of (condi- electrophoresis in polyacrylamide gel. As a result, frac- tional) biological effect. After salting-out, the fractions
Russian Journal of Developmental Biology – Springer Journals
Published: Jun 21, 2005
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