Mammalian Genome 8, 854-856 (1997). 9 Springer-Verlag New York Inc. 1997 James E. Womack, Jan S. Johnson, Elaine K. Owens, Caird E. Rexroad III, Jiirg Schl/ipfer, Ya-Ping Yang Center for Animal Genetics, Institute of Biosciences and Technology, Department of Veterinary Pathobiology, Texas A&M University, College Station, Texas 77843, USA Received: 19 March 1997 / Accepted: 15 June 1997 Although the idea of irradiation and fusion gene transfer was pub- vived. All fusion plates had ten or more colonies growing on day lished more than 20 years ago (Goss and Harris 1975) and em- 8, 101 (7-21 from each plate) of which were picked and cloned ployed in an isolated mapping experiment ten years later (Willard into single wells of 24 well plates containing DMEM, HAT, and et al. 1985), it was not systematically employed as a human gene ouabain. Cells were subsequently transferred to T25 flasks and mapping tool until resurrected by Cox and associates (1990) for maintained on HAT without ouabain. Cells from confluent flasks constructing a high-resolution map of human Chr 21. Whole- were divided to cryopreserve seed stocks, to inoculate roller genome radiation hybrid (WG-RH) mapping utilizing irradiated bottles, and to produce a small pellet for
Mammalian Genome – Springer Journals
Published: Mar 24, 2009
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