A whole-genome radiation hybrid panel for bovine gene mapping

A whole-genome radiation hybrid panel for bovine gene mapping Mammalian Genome 8, 854-856 (1997). 9 Springer-Verlag New York Inc. 1997 James E. Womack, Jan S. Johnson, Elaine K. Owens, Caird E. Rexroad III, Jiirg Schl/ipfer, Ya-Ping Yang Center for Animal Genetics, Institute of Biosciences and Technology, Department of Veterinary Pathobiology, Texas A&M University, College Station, Texas 77843, USA Received: 19 March 1997 / Accepted: 15 June 1997 Although the idea of irradiation and fusion gene transfer was pub- vived. All fusion plates had ten or more colonies growing on day lished more than 20 years ago (Goss and Harris 1975) and em- 8, 101 (7-21 from each plate) of which were picked and cloned ployed in an isolated mapping experiment ten years later (Willard into single wells of 24 well plates containing DMEM, HAT, and et al. 1985), it was not systematically employed as a human gene ouabain. Cells were subsequently transferred to T25 flasks and mapping tool until resurrected by Cox and associates (1990) for maintained on HAT without ouabain. Cells from confluent flasks constructing a high-resolution map of human Chr 21. Whole- were divided to cryopreserve seed stocks, to inoculate roller genome radiation hybrid (WG-RH) mapping utilizing irradiated bottles, and to produce a small pellet for http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Mammalian Genome Springer Journals

A whole-genome radiation hybrid panel for bovine gene mapping

Loading next page...
Copyright © 1997 by Springer-Verlag
Life Sciences; Cell Biology; Anatomy; Zoology
Publisher site
See Article on Publisher Site


You’re reading a free preview. Subscribe to read the entire article.

DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches


Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.



billed annually
Start Free Trial

14-day Free Trial