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Chiann-mun Chen, J. Krohn, S. Bhattacharya, B. Davies (2011)
A Comparison of Exogenous Promoter Activity at the ROSA26 Locus Using a PhiC31 Integrase Mediated Cassette Exchange Approach in Mouse ES CellsPLoS ONE, 6
D. Garrick, S. Fiering, David Martin, E. Whitelaw (1998)
Repeat-induced gene silencing in mammalsNature Genetics, 18
Philippe Soriano (1999)
Generalized lacZ expression with the ROSA26 Cre reporter strainNature Genetics, 21
E. Ryder, B. Doe, Diane Gleeson, Richard Houghton, P. Dalvi, Evelyn Grau, Bishoy Habib, Evelina Miklejewska, Stuart Newman, Debarati Sethi, Caroline Sinclair, Sapna Vyas, H. Wardle-Jones, Joanna Bottomley, J. Bussell, A. Galli, Jennifer Salisbury, R. Ramírez‐Solís (2013)
Rapid conversion of EUCOMM/KOMP-CSD alleles in mouse embryos using a cell-permeable Cre recombinaseTransgenic Research, 23
D. Beier, C. Morton, A. Leder, R. Wallace, P. Leder (1989)
Perinatal lethality (ple): a mutation caused by integration of a transgene into distal mouse chromosome 15.Genomics, 4 4
Jost Seibler, Birgit Küter-Luks, H. Kern, S. Streu, L. Plum, J. Mauer, Ralf Kühn, J. Brüning, F. Schwenk (2005)
Single copy shRNA configuration for ubiquitous gene knockdown in miceNucleic Acids Research, 33
S. Girirajan, Catarina Campbell, E. Eichler (2011)
Human copy number variation and complex genetic disease.Annual review of genetics, 45
Dorothe Hameyer, Ate Loonstra, L. Eshkind, S. Schmitt, Cecilia Antunes, A. Groen, E. Bindels, J. Jonkers, P. Krimpenfort, R. Meuwissen, Loes Rijswijk, A. Bex, A. Berns, E. Bockamp (2007)
Toxicity of ligand-dependent Cre recombinases and generation of a conditional Cre deleter mouse allowing mosaic recombination in peripheral tissues.Physiological genomics, 31 1
Shunsuke Kawamoto, H. Niwa, F. Tashiro, S. Sano, G. Kondoh, J. Takeda, K. Tabayashi, J. Miyazaki (2000)
A novel reporter mouse strain that expresses enhanced green fluorescent protein upon Cre‐mediated recombinationFEBS Letters, 470
M. Klebig, J. Wilkinson, J. Geisler, R. Woychik (1995)
Ectopic expression of the agouti gene in transgenic mice causes obesity, features of type II diabetes, and yellow fur.Proceedings of the National Academy of Sciences of the United States of America, 92 11
A. Nagy, L. Mar, G. Watts (2009)
Creation and use of a cre recombinase transgenic database.Methods in molecular biology, 530
M. Taniguchi, M. Sanbo, S. Watanabe, I. Naruse, M. Mishina, T. Yagi (1998)
Efficient production of Cre-mediated site-directed recombinants through the utilization of the puromycin resistance gene, pac: a transient gene-integration marker for ES cells.Nucleic acids research, 26 2
M. Ohtsuka, Sanae Ogiwara, Hiromi Miura, A. Mizutani, T. Warita, Masahiro Sato, K. Imai, K. Hozumi, Takehito Sato, Masafumi Tanaka, M. Kimura, H. Inoko (2010)
Pronuclear injection-based mouse targeted transgenesis for reproducible and highly efficient transgene expressionNucleic Acids Research, 38
S. Bronson, Elizabeth Plaehn, K. Kluckman, J. Hagaman, Nobuyo Maeda, O. Smithies (1996)
Single-copy transgenic mice with chosen-site integration.Proceedings of the National Academy of Sciences of the United States of America, 93 17
Carolyn Rodriguez, F. Buchholz, J. Galloway, Reynaldo Sequerra, J. Kasper, Ramsés Ayala, A. Stewart, S. Dymecki (2000)
High-efficiency deleter mice show that FLPe is an alternative to Cre-loxPNature Genetics, 25
Miles Miller, D. Duhl, H. Vrieling, S. Cordes, M. Ollmann, B. Winkes, G. Barsh (1993)
Cloning of the mouse agouti gene predicts a secreted protein ubiquitously expressed in mice carrying the lethal yellow mutation.Genes & development, 7 3
Haoyi Wang, Hui Yang, C. Shivalila, Meelad Dawlaty, A. Cheng, Feng Zhang, R. Jaenisch (2013)
One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome EngineeringCell, 153
Kenneth Dobie, Muriel LEEt, Judith FANTESt, Elizabeth GRAHAMt, A. CLARKt, Anthea Springbeti, Richard Lathe, Margaret MCCLENAGHANt (1996)
Variegated transgene expression in mouse mammary gland is determined by the transgene integration locus.Proceedings of the National Academy of Sciences of the United States of America, 93 13
G. Prelich (2012)
Gene Overexpression: Uses, Mechanisms, and InterpretationGenetics, 190
William Kisseberth, Nicholas Brettingen, Jan Lohse, Eric Sandgren (1999)
Ubiquitous expression of marker transgenes in mice and rats.Developmental biology, 214 1
S. Hatada, W. Kuziel, O. Smithies, Nobuyo Maeda (1999)
The Influence of Chromosomal Location on the Expression of Two Transgenes in Mice*The Journal of Biological Chemistry, 274
S. Pettitt, Qi Liang, Xin Rairdan, J. Moran, H. Prosser, D. Beier, K. Lloyd, A. Bradley, W. Skarnes (2009)
Agouti C57BL/6N embryonic stem cells for mouse genetic resourcesNature methods, 6
Y. Lallemand, V. Luria, R. Haffner-Krausz, P. Lonai (1998)
Maternally expressed PGK-Cre transgene as a tool for early and uniform activation of the Cre site-specific recombinaseTransgenic Research, 7
R. Palmiter, R. Brinster (1986)
Germ-line transformation of mice.Annual review of genetics, 20
M. Schaub, A. Boyle, A. Kundaje, S. Batzoglou, Michael Snyder (2012)
Linking disease associations with regulatory information in the human genomeGenome Research, 22
S. Bultman, E. Michaud, R. Woychik (1992)
Molecular characterization of the mouse agouti locusCell, 71
M. Birling, A. Dierich, S. Jacquot, Y. Hérault, G. Pavlovic (2012)
Highly‐efficient, fluorescent, locus directed cre and FlpO deleter mice on a pure C57BL/6N genetic backgroundgenesis, 50
Niwa Hitoshi, Yamamura Ken-ichi, M. Jun-ichi (1991)
Efficient selection for high-expression transfectants with a novel eukaryotic vector.Gene, 108 2
Omar Nyabi, Michael Naessens, Katharina Haigh, Agnieszka Gembarska, S. Goossens, M. Maetens, S. Clercq, Benjamin Drogat, Lieven Haenebalcke, S. Bartunkova, Ilse Vos, Bram Craene, M. Karimi, G. Berx, A. Nagy, P. Hilson, J. Marine, J. Haigh (2009)
Efficient mouse transgenesis using Gateway-compatible ROSA26 locus targeting vectors and F1 hybrid ES cellsNucleic Acids Research, 37
F. Farley, Philippe Soriano, L. Steffen, S. Dymecki (2000)
Widespread recombinase expression using FLPeR (Flipper) micegenesis, 28
C. Chandras, Michael Zouberakis, E. Salimova, D. Smedley, N. Rosenthal, V. Aidinis (2012)
CreZOO—the European virtual repository of Cre and other targeted conditional driver strainsDatabase: The Journal of Biological Databases and Curation, 2012
M. Maurano, R. Humbert, Eric Rynes, R. Thurman, E. Haugen, Hao Wang, Alex Reynolds, R. Sandstrom, H. Qu, Jennifer Brody, A. Shafer, Fidencio Neri, Kristen Lee, T. Kutyavin, Sandra Stehling-Sun, Audra Johnson, T. Canfield, Erika Giste, Morgan Diegel, Daniel Bates, R. Hansen, Shane Neph, P. Sabo, S. Heimfeld, A. Raubitschek, S. Ziegler, C. Cotsapas, N. Sotoodehnia, I. Glass, S. Sunyaev, R. Kaul, J. Stamatoyannopoulos (2012)
Systematic Localization of Common Disease-Associated Variation in Regulatory DNAScience, 337
Christian Hitz, W. Wurst, R. Kühn (2007)
Conditional brain-specific knockdown of MAPK using Cre/loxP regulated RNA interferenceNucleic Acids Research, 35
Ivana Barbaric, G. Miller, T. Dear (2007)
Appearances can be deceiving: phenotypes of knockout mice.Briefings in functional genomics & proteomics, 6 2
A. Bradley, K. Anastassiadis, A. Ayadi, J. Battey, C. Bell, M. Birling, Joanna Bottomley, Steve Brown, Antje Bürger, C. Bult, Wendy Bushell, F. Collins, C. Desaintes, B. Doe, A. Economides, J. Eppig, R. Finnell, R. Finnell, C. Fletcher, M. Fray, D. Frendewey, Roland Friedel, R. Friedel, F. Grosveld, Jens Hansen, Y. Hérault, G. Hicks, A. Hörlein, Richard Houghton, M. Angelis, D. Huylebroeck, V. Iyer, P. Jong, J. Kadin, Cornelia Kaloff, K. Kennedy, M. Koutsourakis, K. Lloyd, S. Marschall, J. Mason, C. McKerlie, M. McLeod, H. Melchner, Mark Moore, A. Mujica, A. Mujica, A. Nagy, M. Nefedov, L. Nutter, G. Pavlovic, J. Peterson, J. Pollock, R. Ramírez‐Solís, D. Rancourt, M. Raspa, J. Remacle, M. Ringwald, B. Rosen, N. Rosenthal, J. Rossant, P. Noppinger, Edward Ryder, J. Schick, F. Schnütgen, P. Schofield, C. Seisenberger, M. Selloum, E. Simpson, W. Skarnes, D. Smedley, D. Smedley, W. Stanford, A. Stewart, Kevin Stone, Kate Swan, Hamsa Tadepally, L. Teboul, G. Tocchini-Valentini, D. Valenzuela, A. West, K. Yamamura, Y. Yoshinaga, W. Wurst, W. Wurst (2012)
The mammalian gene function resource: the international knockout mouse consortiumMammalian Genome, 23
For the analysis of gene function in vivo, gene overexpression in the mouse provides an alternative to loss-of-function knock-out approaches and can help reveal phenotypes where compensatory mechanisms are at play. Furthermore, when multiple lines overexpressing a gene-of-interest at varying levels are studied, the consequences of differences in gene dosage can be explored. Despite these advantages, inherent shortcomings in the methodologies used for the generation of gain-of-function transgenic mouse models have limited their application to functional gene analysis, and the necessity for multiple lines comes at a significant animal and financial cost. The targeting of transgenic overexpression constructs at single copy into neutral genomic loci is the preferred method for the generation of such models, which avoids the unpredictable outcomes associated with conventional random integration. However, despite the increased reliability that targeted transgenic methodologies provide, only one expression level results, as defined by the promoter used. Here, we report a new versatile overexpression allele, the promoter-switch allele, which couples PhiC31 integrase-targeted transgenesis with Flp recombinase promoter switching and Cre recombinase activation. These recombination switches allow the conversion of different overexpression alleles, combining the advantages of transgenic targeting with tunable transgene expression. With this approach, phenotype severity can be correlated with transgene expression in a single mouse model, providing a cost-effective solution amenable to systematic gain-of-function studies.
Mammalian Genome – Springer Journals
Published: Sep 14, 2015
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