A Theiler’s virus BeAn strain shows a persistent profile of BHK-21 cell infection as determined by genome detection using real-time RT-PCR and expression of L* protein

A Theiler’s virus BeAn strain shows a persistent profile of BHK-21 cell infection as determined... Cells from various tissues and species are able to bind to Theiler’s virus strain DA and allow it to replicate to some extent. Meanwhile, permissiveness in vitro to BeAn strains has not been well investigated. In this paper, the BeAn 8386 virus was subjected to five passages in BHK-21 cells and showed a persistent profile. In order to follow the in vitro infection, real-time RT-PCR to detect the IRES, L* and 3A3B regions of the Theiler’s virus genome was carried out in the first and last passages. In addition, the expression of L* protein was detected. These findings confirm the persistence of the virus in vitro , even in the absence of cytopathic effect (CPE). http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

A Theiler’s virus BeAn strain shows a persistent profile of BHK-21 cell infection as determined by genome detection using real-time RT-PCR and expression of L* protein

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Publisher
Springer Vienna
Copyright
Copyright © 2012 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Infectious Diseases; Medical Microbiology
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-012-1434-4
Publisher site
See Article on Publisher Site

Abstract

Cells from various tissues and species are able to bind to Theiler’s virus strain DA and allow it to replicate to some extent. Meanwhile, permissiveness in vitro to BeAn strains has not been well investigated. In this paper, the BeAn 8386 virus was subjected to five passages in BHK-21 cells and showed a persistent profile. In order to follow the in vitro infection, real-time RT-PCR to detect the IRES, L* and 3A3B regions of the Theiler’s virus genome was carried out in the first and last passages. In addition, the expression of L* protein was detected. These findings confirm the persistence of the virus in vitro , even in the absence of cytopathic effect (CPE).

Journal

Archives of VirologySpringer Journals

Published: Dec 1, 2012

References

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