A study of proteins of annexin group in early fish development. IV. Identification of calcium-binding proteins in zebrafish egg by mass spectrometry

A study of proteins of annexin group in early fish development. IV. Identification of... Calcium-binding proteins were isolated from zebrafish (Brachydanio rerio) eggs by the method of precipitation with acidic phospholipids in the presence of calcium ions. The revealed proteins separated by SDS-PAGE were identified by mass spectrometric tryptic peptide fingerprinting. The proteins included annexins A2a, A1a, A13.1, and A5. In addition, copine III, a member of the recently described copine family of C2 domain-containing proteins, was identified. Total RNA was analyzed in mature oocytes by RT-PCR and transcripts of two different annexin A13 forms (A13.1 and A13.2) as well as of annexin A3 were detected. Thus, the presence of both proteins and mRNAs of annexins has been shown in the zebrafish egg. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Developmental Biology Springer Journals

A study of proteins of annexin group in early fish development. IV. Identification of calcium-binding proteins in zebrafish egg by mass spectrometry

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Publisher
SP MAIK Nauka/Interperiodica
Copyright
Copyright © 2008 by MAIK Nauka
Subject
Life Sciences; Animal Anatomy / Morphology / Histology; Developmental Biology
ISSN
1062-3604
eISSN
1608-3326
D.O.I.
10.1134/S1062360408030065
Publisher site
See Article on Publisher Site

Abstract

Calcium-binding proteins were isolated from zebrafish (Brachydanio rerio) eggs by the method of precipitation with acidic phospholipids in the presence of calcium ions. The revealed proteins separated by SDS-PAGE were identified by mass spectrometric tryptic peptide fingerprinting. The proteins included annexins A2a, A1a, A13.1, and A5. In addition, copine III, a member of the recently described copine family of C2 domain-containing proteins, was identified. Total RNA was analyzed in mature oocytes by RT-PCR and transcripts of two different annexin A13 forms (A13.1 and A13.2) as well as of annexin A3 were detected. Thus, the presence of both proteins and mRNAs of annexins has been shown in the zebrafish egg.

Journal

Russian Journal of Developmental BiologySpringer Journals

Published: Jun 13, 2008

References

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