A study of protein profile changes in differentiating human myoblasts

A study of protein profile changes in differentiating human myoblasts The changes in the protein profile in cultured human myoblasts after induction of differentiation were studied by proteomic techniques (a combination of O’Farrell two-dimensional electrophoresis and subsequent protein identification by MALDI-TOF MS and MS/MS analyses). Forty-one proteins have been identified, 25 of which were present in both proliferating and differentiating myoblasts, which allows them to be considered as myoblast housekeeping proteins. The changes in the distribution of some isoforms of tropomyosins, S100 proteins, cofilin, etc. have been revealed. The possible role of these changes in the cell protein profile in the realization of the program of skeletal muscle cell differentiation is discussed. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Developmental Biology Springer Journals

A study of protein profile changes in differentiating human myoblasts

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Publisher
SP MAIK Nauka/Interperiodica
Copyright
Copyright © 2009 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Animal Anatomy / Morphology / Histology; Developmental Biology
ISSN
1062-3604
eISSN
1608-3326
D.O.I.
10.1134/S1062360409020039
Publisher site
See Article on Publisher Site

Abstract

The changes in the protein profile in cultured human myoblasts after induction of differentiation were studied by proteomic techniques (a combination of O’Farrell two-dimensional electrophoresis and subsequent protein identification by MALDI-TOF MS and MS/MS analyses). Forty-one proteins have been identified, 25 of which were present in both proliferating and differentiating myoblasts, which allows them to be considered as myoblast housekeeping proteins. The changes in the distribution of some isoforms of tropomyosins, S100 proteins, cofilin, etc. have been revealed. The possible role of these changes in the cell protein profile in the realization of the program of skeletal muscle cell differentiation is discussed.

Journal

Russian Journal of Developmental BiologySpringer Journals

Published: Apr 6, 2009

References

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