The phase of expression of genes CycB, CycE, and chb were determined in the cell cycle of neuropblasts of D. melanogaster 3rd instar larvae using the previously described radioautographic method and software. CycB was expressed at G 2 phase and upon transition from G 2 phase to M phase, while CycE was expressed at the end of G 1 phase and upon transition from G 1 phase to S phase. The phase of expression of the centrosome-associated protein chb was determined more precisely in G 2 phase. The mean life span of reporter β-galactosidase in neuroblasts was 4 h. The existence of more than one peak of expression of the gene in question in the cell cycle is discussed.
Russian Journal of Developmental Biology – Springer Journals
Published: Oct 13, 2004
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