A Structural Determinant of Desensitization and Allosteric Regulation by Pentobarbitone of the GABAA Receptor

A Structural Determinant of Desensitization and Allosteric Regulation by Pentobarbitone of the... Functional properties of the α1β1 GABAA receptor changes in a subunit-specific manner when a threonine residue in the M2 region at the 12′ position was mutated to glutamine. The rate and extent of desensitization increased in all mutants but the rate of activation was faster in the β1 mutants. A negligible plateau current and abolition of potentiation by pentobarbitone of the GABA-activated current depended on the Thr 12′ Gln mutation being present in the β1 subunit. The Hill coefficient of the peak current response to GABA was reduced to less than one also in a β1 subunit-specific manner. It was concluded that the β1 subunit dominated conformational changes activated by GABA. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Membrane Biology Springer Journals

A Structural Determinant of Desensitization and Allosteric Regulation by Pentobarbitone of the GABAA Receptor

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Publisher
Springer-Verlag
Copyright
Copyright © Inc. by 1997 Springer-Verlag New York
Subject
Life Sciences; Biochemistry, general; Human Physiology
ISSN
0022-2631
eISSN
1432-1424
D.O.I.
10.1007/s002329900167
Publisher site
See Article on Publisher Site

Abstract

Functional properties of the α1β1 GABAA receptor changes in a subunit-specific manner when a threonine residue in the M2 region at the 12′ position was mutated to glutamine. The rate and extent of desensitization increased in all mutants but the rate of activation was faster in the β1 mutants. A negligible plateau current and abolition of potentiation by pentobarbitone of the GABA-activated current depended on the Thr 12′ Gln mutation being present in the β1 subunit. The Hill coefficient of the peak current response to GABA was reduced to less than one also in a β1 subunit-specific manner. It was concluded that the β1 subunit dominated conformational changes activated by GABA.

Journal

The Journal of Membrane BiologySpringer Journals

Published: Jan 15, 1997

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