A simple and sensitive fluorometric dopamine assay based on silica-coated CdTe quantum dots

A simple and sensitive fluorometric dopamine assay based on silica-coated CdTe quantum dots A fluorescence assay is described for the fluorometric determination of dopamine (DA). It based on the use of silica-coated CdTe quantum dots (QD@SiO2). These were fabricated through a hydrothermal process. When DA is added to a solution of the QD@SiO2 and then oxidized by oxygen under catalytic action of tyrosinase to form dopamine quinone, the fluorescence of QD@SiO2 (acquired at excitation/emission wavelengths of 310/525 nm) decreases due to an electron transfer quenching processes. The assay has a linear calibration plot in the 0.05 to 30 μM DA concentration range and a 12.5 nM detection limit (at an S/N ratio of 3). The method was applied to the determination of DA in spiked human serum samples. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Microchimica Acta Springer Journals

A simple and sensitive fluorometric dopamine assay based on silica-coated CdTe quantum dots

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Springer Vienna
Copyright © 2017 by Springer-Verlag Wien
Chemistry; Nanochemistry; Nanotechnology; Characterization and Evaluation of Materials; Analytical Chemistry; Microengineering
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