A search for protein-protein interactions of peroxiredoxin 6 with the yeast two-hybrid system

A search for protein-protein interactions of peroxiredoxin 6 with the yeast two-hybrid system Peroxiredoxins (Prx) are a family of nonselenium peroxidases that are involved in cell defense against oxidative stress and in redox regulation of intracellular signaling. Mammalian peroxiredoxin 6 (Prx6) belongs to the 1-Cys Prx subfamily. The protein-protein interactions of human Prx6 were studied using a yeast two-hybrid system. Hybrid plasmid pHybLex/Zeo/Prx6, which directed synthesis of a chimeric protein consisting of the DNA-binding domain (BD) of LexA and a Prx6 sequence, was used to screen a two-hybrid cDNA library Hybrid Hunter (Invitrogen). The screening identified two potential interaction partners of Prx6: the calcium-activated cysteine endopeptidase calpain and the p50RhoGAP protein of the family of Sec14-like proteins. The possibility for the interactions observed in the two-hybrid system to occur in oxidative stress in vivo is discussed. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Genetics Springer Journals

A search for protein-protein interactions of peroxiredoxin 6 with the yeast two-hybrid system

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SP MAIK Nauka/Interperiodica
Copyright © 2008 by Pleiades Publishing, Ltd.
Biomedicine; Microbial Genetics and Genomics; Human Genetics ; Animal Genetics and Genomics
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