Access the full text.
Sign up today, get DeepDyve free for 14 days.
Koetsier Pa, J. Schorr, W. Doerfler (1993)
A rapid optimized protocol for downward alkaline Southern blotting of DNA.BioTechniques, 15 2
M. Ghanevati, J. Jaworski (2002)
Engineering and mechanistic studies of the Arabidopsis FAE1 beta-ketoacyl-CoA synthase, FAE1 KCS.European journal of biochemistry, 269 14
R. Schiestl, R. Gietz (1989)
High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrierCurrent Genetics, 16
P. Bouvier-Navé, Pierre Benveniste, P. Oelkers, S. Sturley, Hubert Schaller (2000)
Expression in yeast and tobacco of plant cDNAs encoding acyl CoA:diacylglycerol acyltransferase.European journal of biochemistry, 267 1
Aretha Fiebig, J. Mayfield, Natasha Miley, Samantha Chau, R. Fischer, D. Preuss (2000)
Alterations in CER6, a Gene Identical to CUT1, Differentially Affect Long-Chain Lipid Content on the Surface of Pollen and StemsPlant Cell, 12
L. Schreiber, M. Skrabs, K. Hartmann, D. Becker, C. Cassagne, R. Lessire (2000)
Biochemical and molecular characterization of corn (Zea mays L.) root elongases.Biochemical Society transactions, 28 6
(1986)
The promoter
E. Fehling, Kumar Mukherjee (1991)
Acyl-CoA elongase from a higher plant (Lunaria annua): metabolic intermediates of very-long-chain acyl-CoA products and substrate specificity.Biochimica et biophysica acta, 1082 3
L. Kunst, D. Taylor, E. Underhill (1992)
Fatty acid elongation in developing seeds of Arabidopsis thalianaPlant Physiology and Biochemistry, 30
(1983)
Isolation of DNA from higher plants
D. James, E. Lim, J. Keller, Ingrid Plooy, E. Ralston, H. Dooner (1995)
Directed tagging of the Arabidopsis FATTY ACID ELONGATION1 (FAE1) gene with the maize transposon activator.The Plant cell, 7
P. Kolattukudy (1984)
Biochemistry and function of cutin and suberinBotany, 62
L. Kunst, A. Samuels (2003)
Biosynthesis and secretion of plant cuticular wax.Progress in lipid research, 42 1
S. Clemens (1997)
Isolation of a Brassica napus cDNA accession no. AF009563 encoding 3-ketoacyl-CoA synthase, a condensing enzyme involved in the biosynthesis of very long chain fatty acids in seeds (PGR97-125)Plant Physiology, 115
A. Millar, S. Clemens, S. Zachgo, E. Giblin, D. Taylor, L. Kunst (1999)
CUT1, an Arabidopsis Gene Required for Cuticular Wax Biosynthesis and Pollen Fertility, Encodes a Very-Long-Chain Fatty Acid Condensing EnzymePlant Cell, 11
G. Roughan (1994)
A semi-preparative enzymic synthesis of malonyl-CoA from [14C]acetate and 14CO2: labelling in the 1, 2 or 3 position.The Biochemical journal, 300 ( Pt 2)
T.S. Tillman, R.M. Bell (1986)
Mutants of Saccharomyces cerevisiae defective in sn-glycerol-3-phosphate acyltransferaseJ. Biol. Chem., 261
(1982)
Elongase and epicuticular wax biosynthesis
Michael Lassner, K. Lardizabal, James Metz (1996)
A jojoba beta-Ketoacyl-CoA synthase cDNA complements the canola fatty acid elongation mutation in transgenic plants.The Plant cell, 8
(1976)
A rapid and sensitive method of quantitation of microgram quantities of protein
S. Clough, A. Bent (1998)
Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana.The Plant journal : for cell and molecular biology, 16 6
M. Ghanevati, J. Jaworski (2001)
Active-site residues of a plant membrane-bound fatty acid elongase beta-ketoacyl-CoA synthase, FAE1 KCS.Biochimica et biophysica acta, 1530 1
F. Beaudoin, L. Michaelson, S. Hey, Mervyn Lewis, P. Shewry, O. Sayanova, J. Napier (2000)
Heterologous reconstitution in yeast of the polyunsaturated fatty acid biosynthetic pathway.Proceedings of the National Academy of Sciences of the United States of America, 97 12
J. Todd, D. Post-Beittenmiller, J. Jaworski (1999)
KCS1 encodes a fatty acid elongase 3-ketoacyl-CoA synthase affecting wax biosynthesis in Arabidopsis thaliana.The Plant journal : for cell and molecular biology, 17 2
T. Tillman, R. Bell (1986)
Mutants of Saccharomyces cerevisiae defective in sn-glycerol-3-phosphate acyltransferase. Simultaneous loss of dihydroxyacetone phosphate acyltransferase indicates a common gene.The Journal of biological chemistry, 261 20
Matthias Schmidt, J. Feierabend, Ya‐hsuan Hsu, K. To, Chih-Yuan Yang, Yen Lin, J. Shaw, T. Hage, C. Seither, D. Hildebrand, Jun-jun Liu, G. Podila, Frauke Hein, S. Overkamp, W. Barz, K. Nozue, J. Christie, T. Kiyosue, W. Briggs, M. Wada, R. Arredondo-peter, M. Ramírez, G. Sarath, R. Klucas, A. Casas-Mollano, L. Destéfano-Beltrán, G. Coleman, B. Zhu, Sanggyun Park, R. Thornburg, D. Blanchard, A. Esen, E. Or, J. Baybik, Y. Sacks, I. Vilozny, H. Hayashi, N. Hiraoka, Y. Ikeshiro, K. Yazaki, Shigeo Tanaka, T. Kushiro, M. Shibuya, Y. Ebizuka, F. Gubler, Zhongy Li, Sarah Fieg, J. Jacobsen, M. Morell, S. Kojima, S. Hanzawa, T. Hayakawa, M. Hayashi, T. Yamaya, B. Veau, A. Oudin, Martine Courtois, J. Chénieux, S. Hamdi, M. Rideau, M. Clastre, Wen-Joan Chiang, S. Chen, T. Thorbjørnsen, P. Villand, V. Ramstad, L. Kleczkowski, O. Olsen, H. Opsahl-Ferstad, James Anderson, D. Horvath (1995)
The electronic plant gene register.Plant physiology, 120 1
C. Koncz, J. Schell (1986)
The promoter of TL-DNA gene 5 controls the tissue-specific expression of chimaeric genes carried by a novel type of Agrobacterium binary vectorMolecular and General Genetics MGG, 204
A. Millar, L. Kunst (1997)
Very-long-chain fatty acid biosynthesis is controlled through the expression and specificity of the condensing enzyme.The Plant journal : for cell and molecular biology, 12 1
Vesna Katavić, G. Haughn, D. Reed, Marilyn Martin, L. Kunst (1994)
In planta transformation of Arabidopsis thalianaMolecular and General Genetics MGG, 245
R.A. Jefferson (1987)
Assaying chimeric genes in plants: the GUS gene fusion systemPlant. Mol. Biol. Rep., 5
H. Moon, M. Smith, L. Kunst (2001)
A condensing enzyme from the seeds of Lesquerella fendleri that specifically elongates hydroxy fatty acids.Plant physiology, 127 4
E. Cahoon, E. Marillia, K. Stecca, S. Hall, D. Taylor, A. Kinney (2000)
Production of fatty acid components of meadowfoam oil in somatic soybean embryos.Plant physiology, 124 1
M. Bradford (1976)
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Analytical biochemistry, 72
K. Gable, S. Garton, J. Napier, T. Dunn (2004)
Functional characterization of the Arabidopsis thaliana orthologue of Tsc13p, the enoyl reductase of the yeast microsomal fatty acid elongating system.Journal of experimental botany, 55 396
(1989)
High efficiency transformation of intact cells using stranded nucleic acids as carriers
J. Garwin, A. Klages, J. Cronan (1980)
Structural, enzymatic, and genetic studies of beta-ketoacyl-acyl carrier protein synthases I and II of Escherichia coli.The Journal of biological chemistry, 255 24
F. Beaudoin, K. Gable, O. Sayanova, T. Dunn, J. Napier (2002)
A Saccharomyces cerevisiae Gene Required for Heterologous Fatty Acid Elongase Activity Encodes a Microsomal β-Keto-reductase*The Journal of Biological Chemistry, 277
(1995)
Current Protocols in Molecular Biology
Xiaojie Xu, Charles Dietrich, R. Lessire, B. Nikolau, P. Schnable (2002)
The Endoplasmic Reticulum-Associated Maize GL8 Protein Is a Component of the Acyl-Coenzyme A Elongase Involved in the Production of Cuticular Waxes1Plant Physiology, 128
(1997)
Isolation of a Brassica napus cDNA encoding 3-ketoacyl-CoA synthase, a condensing enzyme involved in the biosynthesis of very long chain fatty acids in seeds
The LfKCS45 gene with a high sequence similarity to known 3-ketoacyl-CoA synthases of the mem- brane-bound fatty acid elongase was isolated from Lesquerella fendleri. The LfKCS45 gene has a 1464 bp open reading frame without introns, and is predicted to encode a polypeptide of 487 amino acids with an estimated molecular mass of 54.6 kD. High-stringency DNA blot analysis indicated that there were no closely related genes to LfKCS45 in the L. fendleri genome. Analysis of the fatty acid composition of transformed yeast revealed that expression of the LfKCS45 protein results in the synthesis of two novel very-long-chain fatty acids identified as C28:0 and C30:0. LfKCS45 was found to be not active with acyl-CoA substrates C16 to C24 in length. Reverse transcription-PCR experiments showed that the LfKCS45 gene is expressed only in L. fendleri root tips. Histochemical assays for GUS activity in Arabidopsis transformed with the LfKCS45 promoter-GUS fusion construct confirmed this expression pattern and demonstrated that LfKCS45 transcription is restricted to the cells of the lateral root cap. Abbreviations: PCR, polymerase chain reaction; VLCFA, very-long-chain fatty acid, a fatty acyl group longer than 18 carbons; X:Y, a fatty acyl group containing X carbons with Y double bonds Introduction
Plant Molecular Biology – Springer Journals
Published: Apr 7, 2005
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.