A role for an alternative splice variant of PIF6 in the control of Arabidopsis primary seed dormancy

A role for an alternative splice variant of PIF6 in the control of Arabidopsis primary seed dormancy Phytochrome interacting factor (PIF) transcription factors have been shown to be important in the regulation of seed dormancy and germination by environmental cues. Many PIF-family transcription factors are expressed in seeds but only PIF1 and SPATULA (SPT) have been tested for a role in germination control. Here we show that PIF6 is expressed strongly during seed development, and that two splice variants exist, one full length (the α form), and a second, the β form, in which a cryptic intron containing the potential DNA binding domain is spliced out, predicted to lead to the generation of a premature stop codon. Loss of PIF6 increases primary seed dormancy, whereas overexpression of the β form, but not the α form, reduce dormancy. Our data show the potential for natural splice variants of PIF transcription factors to be important in the evolution of the control of environmental signalling in plants. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

A role for an alternative splice variant of PIF6 in the control of Arabidopsis primary seed dormancy

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Publisher
Springer Netherlands
Copyright
Copyright © 2009 by Springer Science+Business Media B.V.
Subject
Life Sciences; Plant Pathology; Biochemistry, general; Plant Sciences
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1007/s11103-009-9571-1
Publisher site
See Article on Publisher Site

Abstract

Phytochrome interacting factor (PIF) transcription factors have been shown to be important in the regulation of seed dormancy and germination by environmental cues. Many PIF-family transcription factors are expressed in seeds but only PIF1 and SPATULA (SPT) have been tested for a role in germination control. Here we show that PIF6 is expressed strongly during seed development, and that two splice variants exist, one full length (the α form), and a second, the β form, in which a cryptic intron containing the potential DNA binding domain is spliced out, predicted to lead to the generation of a premature stop codon. Loss of PIF6 increases primary seed dormancy, whereas overexpression of the β form, but not the α form, reduce dormancy. Our data show the potential for natural splice variants of PIF transcription factors to be important in the evolution of the control of environmental signalling in plants.

Journal

Plant Molecular BiologySpringer Journals

Published: Nov 13, 2009

References

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