Mammalian Genome 10, 1112–1114 (1999). Incorporating Mouse Genome © Springer-Verlag New York Inc. 1999 A radiation hybrid map of bovine Chromosome 7 and comparative mapping with human Chromosome 19 p arm 1 2 1 Zixia Gu, James E. Womack, Brian W. Kirkpatrick Department of Animal Sciences, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA Department of Veterinary Pathology, Texas A&M University, College Station, Texas 77843, USA Received: 23 April 1999 / Accepted: 20 July 1999 Radiation hybrid mapping is expected to be an efficient and pow- Markers with similar annealing temperature and different size erful strategy in livestock genome mapping. A 5000-rad bovine of PCR products were grouped together to perform duplex PCR. whole-genome radiation hybrid panel has recently been con- Markers were typed with duplex PCR when possible to make structed (Womack et al. 1997), and radiation hybrid maps of bo- efficient use of the radiation hybrid DNA. Each of the 34 markers vine Chromosomes (Chrs) 13, 19, and 23 have been reported with was typed twice to reduce typing error. Markers were retyped if this panel (Schläpfer et al. 1997; Yang et al. 1998; Band et al. there was a discrepancy between results from the first two runs.
Mammalian Genome – Springer Journals
Published: Nov 1, 1999
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